Mutational sceens in Arabidopsis aimed at identifying genes that are required for functionality of the pepper Bs3 restistance gene

拟南芥突变场景旨在鉴定辣椒 Bs3 抗性基因功能所需的基因

• 批准号: 38764569
• 负责人: Professor Dr. Thomas Lahaye
• 金额: --
• 依托单位: Abteilung für Allgemeine Genetik
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/38764569?language=en
• 关键词: Mutational; sceens; Arabidopsis; aimed; identifying

The AvrBs3 protein of the bacterial phytopathogen Xanthomonas campestris pv. vesicatoria (Xcv)is injected via the type III secretion system into the cytoplasm of its host plant pepper. AvrBs3translocates to the host’s nucleus, binds to and transcriptionally activates the promoter of thepepper Bs3 resistance (R) gene, resulting in a hypersensitive response (HR). Bs3 encodes astructurally atypical flavin monooxygenase that does not share homology with any known plantR protein. Preliminary work has shown that the transcription factor AvrBs3 and the “HR executer”Bs3 are functional not only in pepper but also in the model plant Arabidopsis, which is theprerequisite for genetic dissection of the Bs3-mediated immune reaction in Arabidopsis. To do soArabidopsis will be transformed with a reporter construct that contains Bs3 under transcriptionalcontrol of an estradiol inducible promoter. Mutant screens aim to uncover genes that the Bs3protein requires to execute a cell death reaction. After initial characterization of the observedArabidopsis mutants we will isolate the corresponding genes and characterize them at themolecular level. The long-term goal is to elucidate the function of the identified genes in the contextof the Bs3-mediated immune response.

细菌植物病xanthomonas campestris PV的AVRBS3蛋白。 Vesicatoria（XCV）通过III型分泌系统注入其宿主植物胡椒的细胞质中。 AVRBS3 TRANSLANSLANSLANSLECTO宿主的核与Pepper BS3抗性（R）基因的启动子结合并在转录中激活，导致了超敏感的反应（HR）。 BS3编码不与任何已知的Plantr蛋白具有同源性的非典型非典型黄素单加仑酶。初步工作表明，转录因子AVRBS3和“人力资源执行者” BS3不仅在胡椒中，而且在模型植物拟南芥中都起作用，这是BS3介导的拟南芥遗传解剖的先决条件。进行soarabidopsis将通过一个报告构建体进行转换，该报告构建体在雌二醇诱导型启动子的转录control下包含BS3。突变筛选旨在发现BS3蛋白需要执行细胞死亡反应所需的基因。在观察到的拟南芥突变体的初始表征之后，我们将分离相应的基因并在分子水平上表征它们。长期目标是阐明在BS3介导的免疫反应的情况下鉴定基因的功能。