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Executor plant resistance proteins - pathway dissection by forward and reverse genetics

执行植物抗性蛋白 - 通过正向和反向遗传学进行途径剖析

基本信息

批准号：
281354748
负责人：
Professor Dr. Thomas Lahaye
金额：
--
依托单位：
Abteilung für Allgemeine Genetik
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2015
资助国家：
德国
起止时间：
2014-12-31 至 2019-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/281354748?language=en
关键词：
Executor plant resistance proteins pathway

项目摘要

Executor R proteins are a novel, structurally diverse class of proteins that trigger plant immune reactions but that mechanistically differ from well-known NLR-type R proteins. We aim to identify pathway components of executor R proteins to get first insights into their mode of function. We will focus on the analysis of Bs3 and Bs4C, two executor R proteins from pepper. A population of pepper mutant plants will serve as a common platform for complementary forward and reverse genetic screens aimed at identifying components required for Bs3/Bs4C-mediated immune reactions. A forward screen shall identify pathway components by phenotypical screening of the pepper mutant population. Subsequently, causal mutations will be identified by mapping-by-sequencing. In the reverse screen, transcriptome-profiling will identify putative pathway components. These will be functionally studied in corresponding pepper mutant plants identified by TILLING. Once we have identified Bs3/Bs4C pathway components we will clarify how they are involved in the given immune reactions. Our project will provide the first insights into how the executor R proteins Bs3 and Bs4C mediate immune reactions and whether or not distinct executor R proteins and NLR-type R proteins share common signaling components.

Executor R蛋白是一类新颖的、结构多样的蛋白，可触发植物免疫反应，但在机制上不同于众所周知的NLR型R蛋白。我们的目标是确定执行者R蛋白的途径成分，以初步了解它们的功能模式。我们将重点分析辣椒中的两个执行蛋白Bs3和Bs4C。辣椒突变植株群体将作为互补正向和反向遗传筛选的公共平台，旨在识别Bs3/Bs4C介导的免疫反应所需的成分。正向筛选应通过对辣椒突变群体的表型筛选来鉴定途径成分。随后，将通过测序作图来鉴定因果突变。在反向筛选中，转录组分析将识别可能的途径组件。这些将在通过分蘖鉴定的相应辣椒突变植株中进行功能研究。一旦我们确定了Bs3/Bs4C途径的组成部分，我们将阐明它们是如何参与给定的免疫反应的。我们的项目将首次深入了解执行者R蛋白Bs3和Bs4C如何介导免疫反应，以及不同的执行者R蛋白和NLR型R蛋白是否共享共同的信号成分。