Elucidating in planta target genes of Ralstonia solanacearum TALE-like proteins

阐明植物中青枯菌 TALE 样蛋白的靶基因

基本信息

项目摘要

Ralstonia solanacearum, the causal agent of bacterial wilt disease, is a quarantine organism in the USA and EU that ranks among the most devastating microbial pathogens in crop plants. We aim to study the interaction between R. solanacearum and its host plants at the molecular level and anticipate that our findings will enable us, in the long run, to develop effective countermeasures against this devastating pathogen. The effector proteins injected by bacterial pathogens into host cells either promote disease or trigger plant immunity. They are thus key determinants for the outcome of plant-microbe interactions. In the framework of this proposal we will study RipTALs, one class of effectors that are widespread in R. solanacearum and that share homology with Transcription Activator Like Effectors (TALEs) from the bacterial genus Xanthomonas.The TALE code describes the correlation of defined amino acids in a given TALE DNA binding domain with specific DNA bases in its effector binding element (EBE). This allows in silico prediction of EBEs for TALEs of known sequence. In preliminary studies we showed that, like for TALEs, RipTAL EBEs can be predicted with the TALE code. Furthermore RipTALs, like TALEs are injected into host cells and are capable of transcriptionally activating promoters bearing a predicted EBE. It is thus conceivable that RipTALs, like TALEs, transcriptionally activate host genes to promote susceptibility in host plants. Given that R. solanacearum is a root pathogen, while xanthomonads typically infect aerial tissues, it is conceivable that they promote disease by activation of host susceptibility (S) genes that encode functionally distinct proteins.We aim to identify RipTAL activated host S genes that promote bacterial disease when being transcriptionally upregulated by a given RipTAL. Once host S genes have been isolated and functionally validated we want to clarify how S gene products promote R. solanacearum disease at the molecular level.R. solanacearum strains isolated from all over the world can be subdivided into four phylotypes, which correlate with their geographical origin. Notably R. solanacearum phylotypes I, II and IV contain RipTALs that differ in their N- and C-terminal regions and their predicted EBEs. Till now molecular studies of RipTALs have been conducted almost exclusively on RipTALs from phylotype I. In the framework of this proposal we aim to also characterize RipTALs from phylotype II and IV strains with the long term goal to identify and characterize corresponding host S genes and gene products.In summary we aim to identify and characterize host S genes that are activated by RipTALs from diverse R. solanacearum phylotypes. We anticipate that our findings will provide insights into the disease strategies of R. solanacearum, aiding the development of efficient countermeasures to protect crop plants.
青枯雷尔氏菌(Ralstonia solanacearum)是细菌性枯萎病的病原体,在美国和欧盟是检疫性生物,在作物中是最具破坏性的微生物病原体之一。我们的目的是研究R.我们在分子水平上研究了青枯菌及其宿主植物,并预计我们的研究结果将使我们能够从长远来看制定针对这种毁灭性病原体的有效对策。由细菌病原体注入宿主细胞的效应蛋白促进疾病或触发植物免疫。因此,它们是植物-微生物相互作用结果的关键决定因素。在这个建议的框架中,我们将研究RipTAL,一类在R中广泛存在的效应子。青枯菌及其与来自细菌黄单胞菌属的转录激活因子样效应子(TALE)具有同源性。TALE代码描述了给定TALE DNA结合结构域中定义的氨基酸与其效应子结合元件(EBE)中特定DNA碱基的相关性。这允许计算机模拟预测已知序列的TALE的EBE。在初步研究中,我们表明,与TALE一样,RipTAL EBE可以用TALE代码预测。此外,RipTAL,像TALE一样,被注射到宿主细胞中,并且能够转录激活携带预测的EBE的启动子。因此可以想象,RipTAL与TALE一样,转录激活宿主基因以促进宿主植物的易感性。考虑到R.青枯菌是一种根病原体,而黄单胞菌通常感染气生组织,可以想象,它们通过激活宿主易感性(S)基因来促进疾病,这些基因编码功能不同的蛋白质。我们的目标是鉴定RipTAL激活的宿主S基因,这些基因在被给定的RipTAL转录上调时促进细菌疾病。一旦宿主S基因被分离并功能验证,我们希望阐明S基因产物如何促进R。在分子水平上对青枯菌病害进行了研究。从世界各地分离的青枯菌菌株可分为四个类型,这与它们的地理来源有关。特别是R。青枯菌I型、II型和IV型含有RipTAL,其N-和C-末端区域以及其预测的EBE不同。到目前为止,RipTAL的分子研究几乎完全是在RipTAL I型上进行的。在这个提议的框架中,我们的目标也是表征来自RipTAL II型和IV型菌株的RipTAL,长期目标是鉴定和表征相应的宿主S基因和基因产物。青枯菌系统型。我们预计,我们的研究结果将提供深入了解的疾病战略的R。青枯菌,帮助制定有效的对策,以保护作物植物。

项目成果

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Professor Dr. Thomas Lahaye其他文献

Professor Dr. Thomas Lahaye的其他文献

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{{ truncateString('Professor Dr. Thomas Lahaye', 18)}}的其他基金

Manipulation of tomato root cells by Brg11, a TALE-like protein of the bacterial pathogen Ralstonia solanacearum
Brg11(细菌病原体青枯菌的 TALE 样蛋白)对番茄根细胞的操纵
  • 批准号:
    495851523
  • 财政年份:
    2022
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Molecular analysis of virulence and avirulence activities of the bacterial effector protein XopH and related proteins
细菌效应蛋白XopH及相关蛋白毒力和无毒活性的分子分析
  • 批准号:
    418274535
  • 财政年份:
    2019
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Elucidating the molecular basis of effector-triggered host susceptibility mediated by the TAL-like effector Brg11 from the bacterial pathogen Ralstonia solanacearum
阐明细菌病原体青枯菌中 TAL 样效应子 Brg11 介导的效应子触发宿主易感性的分子基础
  • 批准号:
    413908990
  • 财政年份:
    2018
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Elucidating the molecular mechanism of how the executor protein Bs3 from pepper triggers plant cell death
阐明辣椒执行蛋白 Bs3 触发植物细胞死亡的分子机制
  • 批准号:
    388775801
  • 财政年份:
    2017
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Identification of CsLOB1 target genes that promote citrus canker disease
鉴定促进柑橘溃疡病的 CsLOB1 靶基因
  • 批准号:
    326067585
  • 财政年份:
    2016
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Executor plant resistance proteins - pathway dissection by forward and reverse genetics
执行植物抗性蛋白 - 通过正向和反向遗传学进行途径剖析
  • 批准号:
    281354748
  • 财政年份:
    2015
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Isolation and molecular analysis of the pepper Bs4C resistance gene that mediates recognition of the cognate Xanthomonas TAL effector protein AvrBs4
介导同源黄单胞菌 TAL 效应蛋白 AvrBs4 识别的辣椒 Bs4C 抗性基因的分离和分子分析
  • 批准号:
    215184390
  • 财政年份:
    2012
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Mutational sceens in Arabidopsis aimed at identifying genes that are required for functionality of the pepper Bs3 restistance gene
拟南芥突变场景旨在鉴定辣椒 Bs3 抗性基因功能所需的基因
  • 批准号:
    38764569
  • 财政年份:
    2007
  • 资助金额:
    --
  • 项目类别:
    Priority Programmes
Isolierung und molekulare Analyse des Paprika Bs3-Resistenzgens und seiner funktionalen Homologen
辣椒Bs3抗性基因及其功能同源物的分离和分子分析
  • 批准号:
    5452108
  • 财政年份:
    2005
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Isolierung und funktionale Analyse der Resistenzgene Bs3 aus Paprika (Capsicum annuum) und Bs4 aus Tomate (Lycopersicon esculentum)
辣椒(Capsicum annuum)抗性基因 Bs3 和番茄(Lycopersicon esculentum)抗性基因 Bs4 的分离和功能分析
  • 批准号:
    5307874
  • 财政年份:
    2000
  • 资助金额:
    --
  • 项目类别:
    Research Grants

相似国自然基金

农杆菌菌液滴注陆地棉雌蕊柱头的in planta 转化体系优化和转化机理研究
  • 批准号:
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標的遺伝子特異的新規in planta変異創生システムの構築
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The ties that bind: Understanding actin-organelle interactions in planta.
结合的纽带:了解植物中肌动蛋白-细胞器的相互作用。
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    --
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SBIR Phase II: Low-cost in-planta nitrate sensor
SBIR 第二阶段:低成本植物内硝酸盐传感器
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    2155110
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MCA: Understanding cellulose synthase complex in planta using single molecule methods
MCA:使用单分子方法了解植物中的纤维素合酶复合物
  • 批准号:
    2321398
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    23H02190
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通过 iChIP 方法分析干旱胁迫反应以分离植物中的转录复合物
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    22K15137
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UK-Brazil International Partnering Award: Development of novel strategies to address plant-microbes interactions in planta
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    BB/W018659/1
  • 财政年份:
    2022
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    --
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    Research Grant
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