Elucidating in planta target genes of Ralstonia solanacearum TALE-like proteins
阐明植物中青枯菌 TALE 样蛋白的靶基因
基本信息
- 批准号:262924427
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:德国
- 项目类别:Research Grants
- 财政年份:2014
- 资助国家:德国
- 起止时间:2013-12-31 至 2016-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Ralstonia solanacearum, the causal agent of bacterial wilt disease, is a quarantine organism in the USA and EU that ranks among the most devastating microbial pathogens in crop plants. We aim to study the interaction between R. solanacearum and its host plants at the molecular level and anticipate that our findings will enable us, in the long run, to develop effective countermeasures against this devastating pathogen. The effector proteins injected by bacterial pathogens into host cells either promote disease or trigger plant immunity. They are thus key determinants for the outcome of plant-microbe interactions. In the framework of this proposal we will study RipTALs, one class of effectors that are widespread in R. solanacearum and that share homology with Transcription Activator Like Effectors (TALEs) from the bacterial genus Xanthomonas.The TALE code describes the correlation of defined amino acids in a given TALE DNA binding domain with specific DNA bases in its effector binding element (EBE). This allows in silico prediction of EBEs for TALEs of known sequence. In preliminary studies we showed that, like for TALEs, RipTAL EBEs can be predicted with the TALE code. Furthermore RipTALs, like TALEs are injected into host cells and are capable of transcriptionally activating promoters bearing a predicted EBE. It is thus conceivable that RipTALs, like TALEs, transcriptionally activate host genes to promote susceptibility in host plants. Given that R. solanacearum is a root pathogen, while xanthomonads typically infect aerial tissues, it is conceivable that they promote disease by activation of host susceptibility (S) genes that encode functionally distinct proteins.We aim to identify RipTAL activated host S genes that promote bacterial disease when being transcriptionally upregulated by a given RipTAL. Once host S genes have been isolated and functionally validated we want to clarify how S gene products promote R. solanacearum disease at the molecular level.R. solanacearum strains isolated from all over the world can be subdivided into four phylotypes, which correlate with their geographical origin. Notably R. solanacearum phylotypes I, II and IV contain RipTALs that differ in their N- and C-terminal regions and their predicted EBEs. Till now molecular studies of RipTALs have been conducted almost exclusively on RipTALs from phylotype I. In the framework of this proposal we aim to also characterize RipTALs from phylotype II and IV strains with the long term goal to identify and characterize corresponding host S genes and gene products.In summary we aim to identify and characterize host S genes that are activated by RipTALs from diverse R. solanacearum phylotypes. We anticipate that our findings will provide insights into the disease strategies of R. solanacearum, aiding the development of efficient countermeasures to protect crop plants.
在美国和欧盟,青枯病病原菌是一种检疫性微生物,是导致青枯病的病原菌。我们的目标是在分子水平上研究茄青霉与其寄主植物之间的相互作用,并期望我们的研究结果能够使我们在长期内制定有效的对策来对付这种毁灭性的病原体。病原菌将效应蛋白注入宿主细胞,或促进疾病,或触发植物免疫。因此,它们是植物与微生物相互作用结果的关键决定因素。在本提案的框架内,我们将研究riptal,这是一类广泛存在于R. solanacearum中的效应物,与细菌属Xanthomonas的转录激活因子样效应物(Transcription Activator Like effector, TALEs)具有同源性。TALE代码描述了给定的TALE DNA结合域中定义的氨基酸与其效应结合元件(EBE)中的特定DNA碱基的相关性。这允许在计算机上预测已知序列的故事的ebe。在初步研究中,我们发现,像TALE一样,RipTAL ebe可以用TALE代码来预测。此外,像TALEs一样,riptal被注射到宿主细胞中,能够转录激活具有预测EBE的启动子。因此可以想象,riptal和TALEs一样,通过转录激活寄主基因来促进寄主植物的易感性。考虑到茄枯病菌是一种根病原体,而黄单胞菌通常感染地面组织,可以想象它们通过激活宿主易感基因(S)来促进疾病,这些基因编码功能不同的蛋白质。我们的目标是确定RipTAL激活的宿主S基因,当被给定的RipTAL转录上调时,促进细菌疾病。一旦宿主S基因被分离出来并在功能上得到验证,我们想要阐明S基因产物是如何在分子水平上促进茄枯病的。从世界各地分离的茄属真菌可分为4个系型,并与它们的地理来源有关。值得注意的是,龙葵I、II和IV种型在其N端和c端区域及其预测的ebe上含有不同的riptal。到目前为止,riptal的分子研究几乎都是针对i种型的riptal进行的。在本提案的框架内,我们的目标是对II和IV种型菌株的riptal进行表征,长期目标是鉴定和表征相应的宿主S基因和基因产物。综上所述,我们的目的是鉴定和表征来自不同种型的番茄红霉中被riptal激活的宿主S基因。我们期望我们的研究结果将有助于了解茄青霉的病害策略,帮助制定有效的对策来保护作物。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Professor Dr. Thomas Lahaye其他文献
Professor Dr. Thomas Lahaye的其他文献
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{{ truncateString('Professor Dr. Thomas Lahaye', 18)}}的其他基金
Manipulation of tomato root cells by Brg11, a TALE-like protein of the bacterial pathogen Ralstonia solanacearum
Brg11(细菌病原体青枯菌的 TALE 样蛋白)对番茄根细胞的操纵
- 批准号:
495851523 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Research Grants
Molecular analysis of virulence and avirulence activities of the bacterial effector protein XopH and related proteins
细菌效应蛋白XopH及相关蛋白毒力和无毒活性的分子分析
- 批准号:
418274535 - 财政年份:2019
- 资助金额:
-- - 项目类别:
Research Grants
Elucidating the molecular basis of effector-triggered host susceptibility mediated by the TAL-like effector Brg11 from the bacterial pathogen Ralstonia solanacearum
阐明细菌病原体青枯菌中 TAL 样效应子 Brg11 介导的效应子触发宿主易感性的分子基础
- 批准号:
413908990 - 财政年份:2018
- 资助金额:
-- - 项目类别:
Research Grants
Elucidating the molecular mechanism of how the executor protein Bs3 from pepper triggers plant cell death
阐明辣椒执行蛋白 Bs3 触发植物细胞死亡的分子机制
- 批准号:
388775801 - 财政年份:2017
- 资助金额:
-- - 项目类别:
Research Grants
Identification of CsLOB1 target genes that promote citrus canker disease
鉴定促进柑橘溃疡病的 CsLOB1 靶基因
- 批准号:
326067585 - 财政年份:2016
- 资助金额:
-- - 项目类别:
Research Grants
Executor plant resistance proteins - pathway dissection by forward and reverse genetics
执行植物抗性蛋白 - 通过正向和反向遗传学进行途径剖析
- 批准号:
281354748 - 财政年份:2015
- 资助金额:
-- - 项目类别:
Research Grants
Isolation and molecular analysis of the pepper Bs4C resistance gene that mediates recognition of the cognate Xanthomonas TAL effector protein AvrBs4
介导同源黄单胞菌 TAL 效应蛋白 AvrBs4 识别的辣椒 Bs4C 抗性基因的分离和分子分析
- 批准号:
215184390 - 财政年份:2012
- 资助金额:
-- - 项目类别:
Research Grants
Mutational sceens in Arabidopsis aimed at identifying genes that are required for functionality of the pepper Bs3 restistance gene
拟南芥突变场景旨在鉴定辣椒 Bs3 抗性基因功能所需的基因
- 批准号:
38764569 - 财政年份:2007
- 资助金额:
-- - 项目类别:
Priority Programmes
Isolierung und molekulare Analyse des Paprika Bs3-Resistenzgens und seiner funktionalen Homologen
辣椒Bs3抗性基因及其功能同源物的分离和分子分析
- 批准号:
5452108 - 财政年份:2005
- 资助金额:
-- - 项目类别:
Research Grants
Isolierung und funktionale Analyse der Resistenzgene Bs3 aus Paprika (Capsicum annuum) und Bs4 aus Tomate (Lycopersicon esculentum)
辣椒(Capsicum annuum)抗性基因 Bs3 和番茄(Lycopersicon esculentum)抗性基因 Bs4 的分离和功能分析
- 批准号:
5307874 - 财政年份:2000
- 资助金额:
-- - 项目类别:
Research Grants
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