Manipulation of tomato root cells by Brg11, a TALE-like protein of the bacterial pathogen Ralstonia solanacearum

Brg11（细菌病原体青枯菌的 TALE 样蛋白）对番茄根细胞的操纵

基本信息

批准号：
495851523
负责人：
Professor Dr. Thomas Lahaye
金额：
--
依托单位：
Abteilung für Allgemeine Genetik
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2022
资助国家：
德国
起止时间：
2021-12-31 至 2022-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/495851523?language=en
关键词：
Manipulation tomato root cells Brg11

项目摘要

Plant-pathogenic bacteria inject effector proteins into host cells that manipulate key cellular processes to promote disease. Thus, effectors can serve as molecular probes to uncover yet unknown key components of plant-microbe interactions. We study Brg11, an effector protein that is present in numerous strains of the broad-host pathogen Ralstonia solanacearum. Brg11 was found to transcriptionally activate expression of arginine decarboxylase (ADC) genes across all of its host plants, suggesting that ADC proteins promote virulence of R. solanacearum. ADC proteins are rate-limiting enzymes in the biosynthesis of polyamines (PAs), a class of metabolites with two or more amino groups that exist throughout all three kingdoms of life. While R. solanacearum was found to boost host PA levels, strains of the bacterial pathogen Pseudomonas syringae deliberately reduce host PA levels. Notably, elevated PA level inhibit in planta growth of P. syringae but not of R. solanacearum. These observations suggest that R. solanacearum Brg11 induces elevated PA levels in host cells to inhibit growth of microbial niche competitors. We will investigate PA-induced changes in host cells at the cellular and molecular level. Overall, these studies will provide a better understanding of how plant PA levels affect host-microbe interactions.

植物病毒细菌将效应蛋白注入宿主细胞，以操纵关键细胞过程以促进疾病。因此，效应子可以用作分子探针，以发现植物 - 微生物相互作用的关键成分。我们研究BRG11，这是一种效应蛋白，存在于多种宿主病原体Ralstonia solanacearum的菌株中。发现BRG11在其所有宿主植物中都在转录激活精氨酸脱羧酶（ADC）基因的表达，这表明ADC蛋白会促进溶霉菌的毒力。 ADC蛋白是多胺（PAS）的生物合成中的限速酶，这是一类代谢物，具有两个或多个氨基群，它们在生命的所有三个王国中都存在。虽然发现茄菌可提高宿主PA水平，但细菌病原体假单胞菌的菌株故意降低宿主PA水平。值得注意的是，升高的PA水平抑制了丁香假单胞菌的植物生长，但抑制了甲状腺菌的生长。这些观察结果表明，甲状腺菌BRG11诱导宿主细胞中的PA水平升高，以抑制微生物利基竞争者的生长。我们将研究PA诱导的细胞和分子水平的宿主细胞的变化。总体而言，这些研究将更好地了解植物PA水平如何影响宿主微叶相互作用。