Selective Recognition by Nanoparticle Imprinted Thin Films

纳米颗粒压印薄膜的选择性识别

• 批准号: 389619059
• 负责人: Professor Dr. Gunther Wittstock
• 金额: --
• 依托单位: Institut für Chemie (IfC)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/389619059?language=en
• 关键词: Selective; Recognition; Nanoparticle; Imprinted; Thin

Assessing the presence, distribution and toxicological potential of man-made ligand shell-protected nanoparticles (NPs) becomes an increasingly urgent issue as the ability of preparing NPs with very interesting functional properties is tremendously surpassing the ability to predict toxicological hazards of those materials. It is generally accepted that toxicological hazards of nanomaterials stem not only from their elemental composition but to a very important extent from their size, shape and the chemical nature of the ligand shell. Consequently, toxicity testing involves a costly and large variety of assays including animal testing required before NPs can be used for technological application. There are no simple and cost-effective methods available for testing the presence of specific NPs in the environment.This project aims for providing a new, simple and cost-effective approach to selectively detect NPs and predict their interactions with biological materials using nanoparticle-imprinted matrices (NAIM) for NPs speciation. This concept has recently been demonstrated for the first time by the applicant for size discrimination of NPs. A matrix is formed in the presence of template NPs. After the release of the NPs, the imprinted cavities are able to selectively reuptake analyte NPs. Within the project, the approach will be significantly extended to explore selectivity towards different shells. Our work has demonstrated that NAIM should be made of thin films because this allows addressing entrapped NPs by the substrate electrode. Different chemistries will be used to obtain NAIMs in order to provide a large variability of surface groups to detect different ligand shell systems. The approaches include grafting by reduction of functionalized aryldiazonium ions around pre-immobilized NPs and matrices based on substituted poly(phenols) formed around NPs. Of special importance is the exploitation of self-limited growth of such polymers. We will post-functionalize the films in order to enhance the selectivity and to surpress nonspecific adsorption. In order to prevent overgrowing of the templates, we will use insulating NP as templates that are equipped with a shell similar to that of the targeted analyte NPs.Electrooxidation is used as primary mean for the NP detection. The efficiency of template removal will be followed by electrochemistry, scanning electron microscopy (SEM), x-ray photoelectron spectroscopy (XPS) and scanning force microscopy (AFM). The reuptake process will be studied by NP batches distinguished by size, shape and shell. The use of NPs with different cores will allow distinguishing different NP batches after their immobilization in the cavities of the NAIM by surface spectroscopy.

评估人造配体壳保护纳米颗粒（NPs）的存在、分布和毒理学潜力成为一个日益紧迫的问题，因为制备具有非常有趣的功能特性的NPs的能力大大超过了预测这些材料毒理学危害的能力。人们普遍认为，纳米材料的毒理学危害不仅与它们的元素组成有关，而且在很大程度上与它们的大小、形状和配体外壳的化学性质有关。因此，毒性测试涉及成本高昂且种类繁多的分析，包括在NPs用于技术应用之前所需的动物试验。目前还没有一种简单而经济的方法可用于检测环境中特定NPs的存在。该项目旨在提供一种新的、简单的、经济的方法来选择性地检测纳米粒子，并利用纳米粒子印迹矩阵（NAIM）来预测纳米粒子与生物材料的相互作用。这一概念最近首次由NPs的大小歧视申请人证明。在模板np存在的情况下形成矩阵。在NPs释放后，印迹腔能够选择性地再吸收分析物NPs。在项目中，该方法将得到显著扩展，以探索对不同壳层的选择性。我们的工作已经证明，NAIM应该由薄膜制成，因为这允许通过衬底电极定位捕获的NPs。将使用不同的化学物质来获得naim，以便提供大的表面基团可变性来检测不同的配体壳系统。这些方法包括通过在预固定化NPs周围还原功能化芳基重氮离子进行接枝，以及在NPs周围形成基于取代多酚的基质。特别重要的是开发这种聚合物的自限生长。我们将后功能化膜，以提高选择性和抑制非特异性吸附。为了防止模板过度生长，我们将使用绝缘NP作为模板，该模板配备了与目标分析物NP相似的外壳。电氧化被用作NP检测的主要手段。模板去除效率将通过电化学、扫描电子显微镜（SEM）、x射线光电子能谱（XPS）和扫描力显微镜（AFM）来跟踪。再吸收过程将通过大小、形状和外壳区分的NP批次进行研究。使用具有不同核的NP，可以通过表面光谱学在NAIM的空腔中固定不同的NP批次。