Selective Recognition by Nanoparticle Imprinted Thin Films

纳米颗粒压印薄膜的选择性识别

• 批准号: 389619059
• 负责人: Professor Dr. Gunther Wittstock
• 金额: --
• 依托单位: Institut für Chemie (IfC)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/389619059?language=en
• 关键词: Selective; Recognition; Nanoparticle; Imprinted; Thin

Assessing the presence, distribution and toxicological potential of man-made ligand shell-protected nanoparticles (NPs) becomes an increasingly urgent issue as the ability of preparing NPs with very interesting functional properties is tremendously surpassing the ability to predict toxicological hazards of those materials. It is generally accepted that toxicological hazards of nanomaterials stem not only from their elemental composition but to a very important extent from their size, shape and the chemical nature of the ligand shell. Consequently, toxicity testing involves a costly and large variety of assays including animal testing required before NPs can be used for technological application. There are no simple and cost-effective methods available for testing the presence of specific NPs in the environment.This project aims for providing a new, simple and cost-effective approach to selectively detect NPs and predict their interactions with biological materials using nanoparticle-imprinted matrices (NAIM) for NPs speciation. This concept has recently been demonstrated for the first time by the applicant for size discrimination of NPs. A matrix is formed in the presence of template NPs. After the release of the NPs, the imprinted cavities are able to selectively reuptake analyte NPs. Within the project, the approach will be significantly extended to explore selectivity towards different shells. Our work has demonstrated that NAIM should be made of thin films because this allows addressing entrapped NPs by the substrate electrode. Different chemistries will be used to obtain NAIMs in order to provide a large variability of surface groups to detect different ligand shell systems. The approaches include grafting by reduction of functionalized aryldiazonium ions around pre-immobilized NPs and matrices based on substituted poly(phenols) formed around NPs. Of special importance is the exploitation of self-limited growth of such polymers. We will post-functionalize the films in order to enhance the selectivity and to surpress nonspecific adsorption. In order to prevent overgrowing of the templates, we will use insulating NP as templates that are equipped with a shell similar to that of the targeted analyte NPs.Electrooxidation is used as primary mean for the NP detection. The efficiency of template removal will be followed by electrochemistry, scanning electron microscopy (SEM), x-ray photoelectron spectroscopy (XPS) and scanning force microscopy (AFM). The reuptake process will be studied by NP batches distinguished by size, shape and shell. The use of NPs with different cores will allow distinguishing different NP batches after their immobilization in the cavities of the NAIM by surface spectroscopy.

人造配体保护纳米颗粒（NP）的存在，分布和毒理潜力成为一个日益紧迫的问题，因为准备具有非常有趣的功能特性的NP的能力是超越预测这些材料毒理学危害的能力。人们普遍认为，纳米材料的毒理危害不仅源于其元素组成，而且源于其大小，形状和配体壳的化学性质的重要程度。因此，毒性测试涉及可以将NPS之前的动物测试（包括在技术应用中使用）的昂贵和大量测定。没有简单且具有成本效益的方法可用于测试环境中特定NP的存在。该项目旨在提供一种新的，简单且具有成本效益的方法来选择性地检测NP，并使用纳米颗粒型矩阵（NAIM）预测其与生物材料的相互作用。最近，申请人首次证明了这一概念的NPS大小歧视。在模板NP的存在下形成矩阵。 NP释放后，印迹的腔体能够选择性地重新摄取分析物NP。在项目中，该方法将大大扩展以探索对不同壳的选择性。我们的工作表明，NAIM应该由薄膜制成，因为这允许通过基板电极夹住的NP来解决。不同的化学物质将用于获取NAIMS，以提供表面基团的巨大变化，以检测不同的配体壳系统。这些方法包括通过基于围绕NP形成的取代的聚（苯酚）围绕预击的NP和矩阵来减少官能化的Aryldiazonium离子进行嫁接。特别重要的是剥削此类聚合物的自限生长。我们将在锻炼后进行功能，以增强选择性并散发非特异性吸附。为了防止模板的过度生长，我们将使用绝缘NP作为配备壳的模板，这些模板与靶向分析物NP相似。Electroo氧化用作NP检测的主要平均值。删除模板的效率将随后进行电化学，扫描电子显微镜（SEM），X射线光电光谱（XPS）和扫描力显微镜（AFM）。重新摄取过程将通过以大小，形状和外壳为特色的NP批次研究。通过表面光谱固定在NAIM的腔内后，使用具有不同核心的NP将允许区分不同的NP批次。