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Reconstruction of the phylogeny of anaerobic chytridiomycetes (Neocallimasticales, Fungi) based on multigene-genealogie

基于多基因谱系重建厌氧壶菌（Neocallimasticales，Fungi）的系统发育

基本信息

批准号：
39071928
负责人：
Privatdozentin Dr. Kerstin Voigt, Ph.D.
金额：
--
依托单位：
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2007
资助国家：
德国
起止时间：
2006-12-31 至 2010-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/39071928?language=en
关键词：
Reconstruction phylogeny anaerobic chytridiomycetes Neocallimasticales

项目摘要

Anaerobic gut fungi are evolutionarily extraordinary in the fungal kingdom. Unique traits of gut fungi especially their anaerobiosis, possession of hydrogenosomes instead of mitochondria, multiple flagella and extremely low genomic GC content caused considerable difficulties with their classification within the class of the chytridiomycetes. Modern systematics of anaerobic fungi based on DNA analyses eliminates the problems with extensive morphological variation of gut fungi depending on environmental conditions. However molecular data based on ribosomal DNA sequences did not answered to satisfaction the relationship between aerobic and anaerobic chytrids because of the repetitive and heterogenous nature of the ribosomal DNA cluster. Molecular analyses of multiple protein-coding genes overcome these problems and offer a robust strategy to study the phylogenetic position of anaerobic fungi among other fungal groups. Single copy protein-coding genes encoding actin, ß- tubulin, translation elongation factor 1-ot or genes encoding enzymes involved in chitin degradation and energy metabolism undergo strong selection and are reliable markers for the reconstruction of phytogenies. The present research proposal results in the generation of a five-gene-genealogy from all taxonomic groups of the chytridiomycetes in comparison with major clades of other fungal lineages. Since animals and fungi are known to be sister groups and flagellate fungi are accepted to represent the most basal lineage of fungi our project will shed light on the ancient evolutionary history of divergence between animals and fungi.

厌氧肠道真菌在真菌王国中进化非凡。肠道真菌的独特特征，特别是它们的厌氧性，拥有氢化酶体而不是线粒体，多鞭毛和极低的基因组GC含量造成了相当大的困难，它们在壶菌纲中的分类。基于DNA分析的厌氧真菌的现代系统学消除了肠道真菌依赖于环境条件的广泛形态变化的问题。然而，基于核糖体DNA序列的分子数据没有回答满意的好氧和厌氧壶菌之间的关系，因为重复和异质性的核糖体DNA簇。多个蛋白质编码基因的分子分析克服了这些问题，并提供了一个强大的战略，以研究其他真菌群中的厌氧真菌的系统发育位置。编码肌动蛋白、微管蛋白、翻译延伸因子1-ot的单拷贝蛋白质编码基因或编码参与几丁质降解和能量代谢的酶的基因经历强选择，并且是重建植物发生学的可靠标记。目前的研究建议的结果在生成一个五基因谱系的所有分类组的壶菌与其他真菌谱系的主要分支相比。由于动物和真菌被认为是姐妹群，而鞭毛虫真菌被认为是真菌最基本的谱系，我们的项目将揭示动物和真菌之间分歧的古老进化史。