Function of CDPK activation, localization and interaction with regulatory proteins during the induction of plant defence responses

钙依赖性蛋白激酶（Ca 2 + dependent protein kinases，CDPK）是一类对植物和某些原生生物具有特异性的丝氨酸/苏氨酸蛋白激酶基因家族，其钙离子传感器结构域与蛋白激酶效应器结构域直接相连。CDPKs被认为参与了环境生物和非生物信号诱导的胁迫反应的激活。然而，仍然很少有信息已可用于不同的CDPK异构体的生物学功能和它们在体内的生化调节。我们在SPP 1212的关键问题是调查是否在并行MAP激酶信号传导也是钙依赖性的信号传导途径介导的CDPKs是必需的重编程和激活的早期防御反应触发先天性免疫在植物中。我们的目标是（1）从A. thaliana中，并研究它们的生物化学活化和它们在病原体和PAMP效应子诱导的防御反应中的作用，以及（2）评估CDPK信号传导是否代表微生物效应子蛋白对病原体介导的防御抑制的潜在靶标。

Calcium-dependent protein kinases (CDPK) are a gene family of serine/threonine protein kinases specific to plants and some protists, in which the regulatory calcium-binding sensor domain is directly linked to the protein kinase effector domain. CDPKs have been discussed to participate in the activation of environmental biotic and abiotic signal-induced stress responses. However, still little information has been available about the biological function of distinct CDPK isoforms and their biochemical regulation in vivo. Our key question within SPP1212 is to investigate whether in parallel to MAP kinase signalling also a calcium-dependent signalling pathway mediated by CDPKs is required for the reprogramming and activation of early defence responses triggering innate immunity in plants. Our objectives are (1) to identify single candidate CDPK isoforms from A. thaliana and investigate their biochemical activation and their role in pathogen and PAMP effector-induced defence responses, and (2) to assess whether CDPK signalling represents a potential target for pathogen-mediated defence suppression by microbial effector proteins.