Biochemical regulation and function of CDPKs in the calcium-regulated abiotic stress signalling network

钙依赖性蛋白激酶（CDPK）是丝氨酸/苏氨酸蛋白激酶，其中钙结合传感器结构域直接连接到一个分子内的蛋白激酶效应结构域。我们的目的是研究CPK 21和CPK 23在早期非生物胁迫反应发生期间细胞内钙调节网络中的贡献。在第一部分中，我们将比较分析CPK 21和CPK 23的体内功能和磷酸化和钙结合的生化激活，以及CPK 21和CPK 23整合到ABA感知信号复合物中。在第2部分中，我们将解决CDPK信号网络：这包括离子通道，转运蛋白或RCAR家族成员之间的潜在磷酸化靶点的识别，以及在非生物胁迫的背景下与CDPK或CIPK类蛋白激酶或磷酸酶的进一步成员的共调节。

Calcium-dependent protein kinases (CDPK) are serine/threonine protein kinases in which the calcium-binding sensor domain is directly joined to a protein kinase effector domain within one molecule. Our objective is to investigate the contribution of CPK21 and CPK23 in the intracellular calcium-regulatory network during the onset of early abiotic stress responses. In part 1, we will perform a comparative analysis of CPK21 and CPK23 in vivo function and biochemical activation by phosphorylation and calcium-binding, and the integration of CPK21 and CPK23 into ABA-perception signalling complexes. In part 2 we will address the CDPK signalling network: this includes the identification of potential phosphorylation targets among ion channels, transporters or members of the RCAR family and the co-regulation with further members of the CDPK or CIPK class of protein kinases or phosphatases in the context of abiotic stress.