Structure and Regulation of the genecluster for CO_2-fixation from Microorganisms

微生物固定CO_2基因簇的结构和调控

• 批准号: 05454070
• 负责人: KODAMA Tohru
• 金额: $ 4.42万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454070/
• 关键词: RubisCO; Hydrogen bacteria; Regulation of geme expression; Pseudomonas; Hydrogenovibrio

Pseudomonas hydrogenothermophila and Hydrogenovibrio marinus are the fastest growing CO_2-fixing organisms among all kind of living matters. Both microorganisms fix CO_2 via the Calvin-Benson Cycle. The genetic studies on the CO_2 assimilation by these organisms have been achieved.RubisCO genes (cbbLS) of Pseudomonas hydrogenothermophila have been cloned and sequenced. We could not find a regulatory gene as cbbR at the upstream of cbbLS,but the existence of 2 open reading flames (cbbQ and cbbO) was confirmed at the downstream of cbbLS.Genetic studies strongly suggested both of CbbQ and CbbO function for the assembly and/or stabilization of CbbLS.Hydrogenovibrio marinus contains two L8S8-type (CbbLS-1, CbbLS-2) and one L2-type (CbbM) RubisCOs in the cells. The strain seems to adapt natural environment by changing the pattern of the expression of three RubisCOs with different characteristics.

嗜热假单胞菌和海洋产氢弧菌是各类生物中生长最快的固碳微生物。这两种微生物都通过卡尔文-本森循环固定CO_2。对嗜热假单胞菌吸收二氧化碳的遗传学进行了研究，克隆了嗜热假单胞菌RubisCO基因(CbbLS)，并对其进行了序列测定。我们没有在cbbLS的上游找到cbbR这样的调控基因，但在cbbLS的下游存在两个开放阅读火焰(cbbQ和cbbO)。遗传学研究有力地表明，CbbQ和CbbO都具有组装和/或稳定CbbLS的功能。该菌株似乎通过改变三种不同特性的RuBisCOs的表达模式来适应自然环境。

项目成果

Toshiaki YAGUCHI,et al.: "Cloning and Soquence of L_2-form RubisCO from Hydrogenovibrio marinus MH-110" Biosci.Biotech.Biochem.,. 58. 1733-1737 (1994)

Toshiaki YAGUCHI 等人：“来自海藻氢弧菌 MH-110 的 L_2 型 Rub​​isCO 的克隆和序列”Biosci.Biotech.Biochem.,。

M.Ishii et.al.: "Electron Transport System in Pseudomonas hydrogenothermophila strain TH-121GC04:Biosci.Biotech.Biochem." 58. 1-4 (1994)

M.Ishii 等人：“嗜热氢假单胞菌菌株 TH-121GC04 中的电子传输系统：Biosci.Biotech.Biochem。”

H.Nishihara et al: "Production and Properties of Glycogen in Hydrogenovibrio marinus." J.Ferment.Bioeng.75. 414-416 (1993)

H.Nishihara 等人：“海藻氢弧菌中糖原的产生和特性。”

S.Y.Chung et al: "Purification and Some Properties of RubisCO from Pseudomonas hydrogenothermophila strain TH-1." J.Ferment.Bioeng.78. 469-471 (1994)

S.Y.Chung 等人：“来自嗜热氢假单胞菌菌株 TH-1 的 RubisCO 的纯化和一些特性。”

Masaharu ISHII,et.al.: "Electron transport System in Pseudomonas hydrogenothermophila StrainTH-1." Biosci.Biotech.Biochem.58. 1-4 (1994)

Masaharu ISHII 等人：“嗜氢假单胞菌菌株 TH-1 中的电子传输系统”。