Carbon Dioxide Fixation in Extreme Environment

极端环境下的二氧化碳固定

• 批准号: 03453136
• 负责人: KODAMA Tohru
• 金额: $ 4.42万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03453136/
• 关键词: CO_2-fixation; RubisCO; Thermophile; marine bacteria; glycogen; halophile; 好アルカリ菌; 古細菌; 海洋性細菌; 好熱性藻類; RuBisCO

Hydrogenovibrio marinus, a fast-growing CO_2-fixing marine bacterium produced significant amount of glycogen in the cells, when grown under the nitrogen limitting condition. The strain was shown to posess 3 RubisCO genes. At least 2 of them were expressed in H.marinus cells.Thermophilic microalgae were isolated from hotspring in Japan and Thailand, and taxonomic studies and improvement of cultivation procedure were achieved on the strains. The RubisCO gene of one of the obtained strains, Synechococcus strain a-1, was cloned and expressed in E.coli cells.Alcalophilic bacteria grown on formic acid were isolated from soil. RubisCO was purified from one of the isolated strains, which was identified as Moraxlla sp. The amino acid sequence of the N-terminal region of the purified RubisCO was similar to that of Alcaligenes eutrophus. But, kinetic studies revealed that Km value for CO_2 of RubisCO from Moraxlla sp.is about 10-fold higher than that from A.eutrophus.Halophilic bacteria, expecially Halofelux strains, were proved to posess distinct ability to fix CO_2 in heterotrophic culture condition.

海洋氢化弧菌（Hydrogenovibrio marinus）是一种生长迅速的海洋CO_2固定菌，在氮限制条件下生长，细胞内可产生大量糖原。该菌株具有3个RubisCO基因。从日本和泰国的温泉中分离到嗜热微藻，并对其进行了分类学研究和培养方法的改进。克隆了其中一株聚球藻a-1的RubisCO基因，并在大肠杆菌中表达。从其中一株分离菌株中纯化了RubisCO，鉴定为莫拉氏菌属。纯化的RubisCO的N-末端区域的氨基酸序列与真养产碱杆菌的氨基酸序列相似。但动力学研究表明，产自莫拉氏菌的RubisCO对CO_2的Km值比产自真养曲霉的高10倍左右，嗜盐菌，特别是嗜盐菌（Halofelux）在异养培养条件下具有明显的固定CO_2的能力。sp.is

项目成果

Hirofumi Nishihara: "Growth Characferistics of a Marine Hydrogen-Oxidising Bacterian Hydrogenou moすinus MH-110" J.Ferment.Bioeng.72. 358-361 (1991)

Hirofumi Nishihara：“海洋氢氧化细菌 Hydrogenou mosuinus MH-110 的生长特征”J.Ferment.Bioeng.72 (1991)。

五十嵐 泰夫,児玉 徹(分担執筆): "極限環境微生物ハンドブック:§6ー2炭酸固定と利用pp376ー384" サイエンス・フォ-ラム社, 467 (1991)

Yasuo Igarashi、Toru Kodama（合著者）：“极端微生物手册：§6-2 碳酸固定和利用 pp376-384” 科学论坛出版，467（1991）

Toshiaki Yaguchi: "Cloning, Sequence and Overexpression of the Thermophilic Cyanobacterium for RubisCO." J.Ferment.Bioeng.75. 1-8 (1994)

Toshiaki Yaguchi：“RubisCO 嗜热蓝细菌的克隆、测序和过度表达。”

Toshiaki Yaguchi: "Cloning,Sequence and Overexpression of the Thermophilic Cyanobacteriam for RubisCO" J.Ferment.Bioeng.75. 1-8 (1993)

Toshiaki Yaguchi：“RubisCO 嗜热蓝藻的克隆、序列和过度表达”J.Ferment.Bioeng.75。

Hirofumi NISHIHARA: "Growth Characteristics of a Marine HydrogenーOxidizing Bacteriam,Hydrogenovibrio marinus MHー110 ander a Continuoas GasーFlow System." J.Ferment.Bioeng. 72. 358-361 (1991)

Hirofumi NISHIHARA：“海洋氢氧化细菌、Hydrogenovibrio marinus MH-110 和连续气流系统的生长特性。”J.Ferment.Bioeng。