Studies on the microbiological basic technologies for bioremediation

生物修复微生物基础技术研究

• 批准号: 05304051
• 负责人: KODAMA Tohru
• 金额: $ 5.06万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05304051/
• 关键词: Bioremediation; Genetical breeding; Bioreclamation of soils; Trichloroethylene; PCB degradation; Reduction of heavy metals; Denitrification; Degradation enzyme genes; 遺伝子のクローニング; 脱窒関連酵素

Through our research works performed during last year, the concept for "Bioremediation" was made clear, and the present state of the worldwide research development was fully understood. On the basis of these results, the microbiological research subjects for the practical bioremediation were investigated and discussed. Some of our members participated and presented the papers in the "OECD Workshop on Bioremediation Tokyo'94", held in November 1994. The effectiveness and safety as well as the necessity of international cooperation for the application of biotechnology for encironmental crises were discussed and confirmed at the workshop. Fundamental researches for the microbial degradation of BHC,chlorinated aromatic hydrocarbons, TCE,PEG and other recalcitrant compounds were developed. The degrading enzyme genes were analyzed and further investigated for enhancing the gene functions for the purpose of their practical use for bioremediation. Members belonging to private companies exploited the supporting technologies to establish the practical bioremediation, for example, improvement of the apparatus for effective treatment of polluted soil or waste water. The results of the present research works will be published by a publisher, Gihodo Shuppan Co.Ltd., in May 1995 in Japanese under the title "Various Kinds of Microorganisms Preserving the Environment".

通过我们去年的研究工作，明确了“生物修复”的概念，并充分了解了世界范围内的研究发展现状。在此基础上，对实际生物修复中的微生物学研究课题进行了探讨。我们的一些成员参加了1994年11月举行的“经合组织94年东京生物修复讲习班”并提交了论文。研讨会讨论并确认了生物技术应用于环境危机的有效性和安全性以及国际合作的必要性。开展了微生物降解六六六、氯代芳烃、三氯乙烯、聚乙二醇等难降解化合物的基础研究。对降解酶基因进行了分析，并进一步研究了提高基因功能的目的，其实际应用的生物修复。属于私营公司的成员利用辅助技术建立实际的生物补救，例如改进有效处理受污染土壤或废水的设备。本研究工作的结果将由出版商Gihodo Shuppan Co.Ltd.出版，1995年5月在日本出版，标题为“各种微生物保护环境”。

项目成果

Ohshima,T.: "Cloning and sequencing of a gene encoding nitrite reductase from Paracoccus denitrificans and expression of the gene in Escherichia coli" J.Ferment.Bioeng.76. 82-88 (1993)

Ohshima,T.：“脱氮副球菌亚硝酸还原酶编码基因的克隆和测序以及该基因在大肠杆菌中的表达”J.Ferment.Bioeng.76。

Kawai,F.et al.: "Proposed mechanism for bacterial degradation of polyacrylate" J.Environ.Polym.Degr.2. 59-65 (1994)

Kawai,F.et al.：“聚丙烯酸酯细菌降解的提议机制”J.Environ.Polym.Degr.2。

Konohana, T.et al.: "Nitrate reduction in Bacillus licheniformis in the presence of ammonium salt by shaking culture" Biosci.Biotech.Biochem.57. 1082-1086 (1994)

Konohana, T. 等人：“在存在铵盐的情况下，通过摇动培养物减少地衣芽孢杆菌中的硝酸盐”Biosci.Biotech.Biochem.57。

Kato,J.et al.: "Cloning and characterization of a Pseudomonas aeruginosa gene involved in the negative regulation of phosphate tais" J.Bacteriol.176. 5874-5877 (1994)

Kato,J.et al.：“参与磷酸盐负调控的铜绿假单胞菌基因的克隆和表征”J.Bacteriol.176。

Furukawa,K.: "Gene components responsible for discrete substrate specificity in the catabolism of biphenyl(bph operon)and toluene(tod operon)" J.Bacteriol.175. 5224-5232 (1993)

Furukawa,K.：“负责联苯（bph 操纵子）和甲苯（tod 操纵子）分解代谢中离散底物特异性的基因组件”J.Bacteriol.175。