Artificial control of oocyte maturation by in vitro perfused organ culture of the ovaries of farm animals

家畜卵巢体外灌注器官培养人工控制卵母细胞成熟

• 批准号: 05454112
• 负责人: MIYAMOTO Hajime
• 金额: $ 4.29万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454112/
• 关键词: Goat; Ovary; Physiological properties; Histochemistry; Oocytes maturation; In vitro culture; Enzyme; Steroid hormone; 卵巣; 灌流培養; 培養液; 卵; 卵胞; 黄体; 脱水素酵素; 潅流培養; グルコース量; 乳酸量; 3β-水酸基脱水素酵素; グルコース-6リン酸脱水素酵素; 排卵

In the present investigation, the operation techniques for in vitro perfused organ culture of caprine ovaries was established. Physiological properties of the goat ovaries with cultured by the perfusion technique were estimated biochemically and histochemically. More than 60% ovaries investigated in the present study showed stigma of Graffian follicles such phenomenon occurs before physiologically normal ovulation in vivo. No rupture during the perfusion was observed. Although the thecal layrs appeared somewhat edematous, ovarian structure was well preserved. In the results of assessing the histochemical activities of enzymes relating steroid hormone synthesis, no difference of the enzyme reactions between before and after perfusion culture was observed. In conclusion, our novel techniques preserve the physiological properties of caprine ovaries during in vitro organ culture by the perfusion technique.

本研究建立了山羊卵巢体外灌注器官培养的操作技术。用生物化学和组织化学方法对经灌注培养的山羊卵巢的生理特性进行了评价。本研究调查的60%以上的卵巢存在格拉夫卵泡柱头现象，这种现象发生在体内生理正常排卵之前。灌注过程中未见破裂。虽然鞘层有些水肿，但卵巢结构保存完好。在评估类固醇激素合成相关酶的组织化学活性的结果中，灌注培养前后的酶反应没有差异。总之，我们的新技术通过灌注技术在体外器官培养过程中保持了山羊卵巢的生理特性。

项目成果

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Miyamoto H.等人：“衰老加速小鼠品系的雌性生殖特性和产前发育”《实验动物学杂志》。

Inoue M. et al: "Accumulation of the c-fos protein in maturing oocytes in Graffian follicles in mice" J. Reprod. Dev.39. 243-249 (1993)

Inoue M. 等人：“小鼠 Graffian 卵泡中成熟卵母细胞中 c-fos 蛋白的积累”J. Reprod。

Miyamoto H.et al.: "A morphometric study of spermatogenesis in the testes of mice of a senescence accelerated strain" Experientia. 50. 808-811 (1994)

Miyamoto H.等人：“衰老加速品系小鼠睾丸中精子发生的形态测量研究”Experientia。

Miyamoto et al.: "Histochemical studies in the perfused goat ovaries" J.Jpn.Embryo Trans.17. 81-87 (1995)

Miyamoto 等人：“灌注山羊卵巢的组织化学研究”J.Jpn.Embryo Trans.17。

Manabe N. et al.: "Immunohistochemical quantitation for extracellular matrix proteins in rats with glomerulonephritis" Nephron. 71. 79-86 (1995)

Manabe N. 等人：“肾小球肾炎大鼠细胞外基质蛋白的免疫组织化学定量”肾单位。