Artificial control of oocyte maturation by in vitro perfused organ culture of the ovaries of farm animals

本研究建立了山羊卵巢体外灌注器官培养的操作技术。用生物化学和组织化学方法对经灌注培养的山羊卵巢的生理特性进行了评价。本研究调查的60%以上的卵巢存在格拉夫卵泡柱头现象，这种现象发生在体内生理正常排卵之前。灌注过程中未见破裂。虽然鞘层有些水肿，但卵巢结构保存完好。在评估类固醇激素合成相关酶的组织化学活性的结果中，灌注培养前后的酶反应没有差异。总之，我们的新技术通过灌注技术在体外器官培养过程中保持了山羊卵巢的生理特性。

In the present investigation, the operation techniques for in vitro perfused organ culture of caprine ovaries was established. Physiological properties of the goat ovaries with cultured by the perfusion technique were estimated biochemically and histochemically. More than 60% ovaries investigated in the present study showed stigma of Graffian follicles such phenomenon occurs before physiologically normal ovulation in vivo. No rupture during the perfusion was observed. Although the thecal layrs appeared somewhat edematous, ovarian structure was well preserved. In the results of assessing the histochemical activities of enzymes relating steroid hormone synthesis, no difference of the enzyme reactions between before and after perfusion culture was observed. In conclusion, our novel techniques preserve the physiological properties of caprine ovaries during in vitro organ culture by the perfusion technique.