Efficient use of oocytes by the perfusion of farm animal ovary

为了有效利用卵母细胞和检测卵巢功能，研究了山羊卵巢体外灌注技术。研究了不同介质灌注对卵巢功能的影响，特别是对卵泡破裂和肿胀现象的影响。建立了山羊卵巢离体灌注系统。灌注装置由具有三个主要部分的再循环系统组成：氧合器、滚子泵和灌注室。灌注卵巢的功能完整性证实了灌注液的pH值，葡萄糖和乳酸浓度的系列测定，并通过组织学和组织化学的ovari.After几个初步的实验，九个实验，其中卵巢含有Graafian卵泡，进行了尝试。在9个实验中的一个实验中，通过灌注山羊卵巢发生了两个Graafian卵泡破裂。但在其他实验中，Graafian卵泡未发生破裂。灌注O ...更多信息 f卵巢引起Graafian卵泡和小窦卵泡肿胀，伴有间质水肿。在灌注系统中观察到的Graafian卵泡破裂可能是伪影，需要仔细选择灌注介质以避免误解。在6小时的灌注期间，葡萄糖消耗和乳酸产生持续发生。光学显微镜显示，尽管在灌注的卵巢中观察到间质水肿形成，但在整个灌注过程中几乎保持了正常的卵巢结构。在组织化学研究中，3 β-HSD和G6 PDH的正常活性在灌注卵巢的卵泡内膜和颗粒细胞中保持。电镜观察结果表明，目前的卵巢灌注系统不能很好地维持卵巢组织，因此，如果能改进灌注技术，建立一种家畜卵巢灌注系统将有助于卵母细胞的有效利用和卵巢功能的研究。少

A technique for in vitro perfusion of goat ovaries is studied to use oocytes efficiently and to examine ovarian function. The effect of perfusion with different media on ovarian function and, in particular, on the phenomenon of follicular rupture and swelling is examined. A system has been developed for the perfusion of the goat ovary in vitro. The perfusion apparatus consisted of a recycling system with three major portions: an oxygenator, a roller pump, and a perfusion chamber. The functional integrity of the perfused ovary is confirmed by serial determinations of the perfusate pH, glucose and lactate concentrations, and by histology and histochemistry of the ovary.After several preliminary experiments, nine experiments, where the ovary containing Graafian follicles was used, were tried. In one of nine experiments the rupture of two Graafian follicles occurred by perfusion of the goat ovary. However in the other experiments the rupture of Graafian follicles did not occur. Perfusion o … More f the ovary caused swelling of Graafian follicles and small antral follicles, accompanied by a stromal edema. Graafian follicular rupture observed in perfusion system may be artifacts and careful selection of the perfusion medium is needed to avoid misinterpretation.Glucose consumption and lactate production occurred continuously during the 6-hour perfusion period. It was shown by light microscopy that almost normal ovarian structure was maintained throughout the perfusion, although interstitial edema formation was noted in the perfused ovaries. In the histochemical study, normal activity of 3beta-HSD and G6PDH was maintained in the theca interna and granulosa cells of follicles in the perfused ovaries. In contrast, electron microscopic observations indicated that the present perfusion system is not satisfactory to maintain ovarian tissue.In conclusion, a perfusion system of the farm animal ovary is useful for the efficient use of oocytes and for the study of ovarian function if perfusion technique could be improved. Less