Regulation mechanisms of germinal cell selection in mammals and its artificial control

哺乳动物生殖细胞选择的调控机制及其人工控制

• 批准号: 11460127
• 负责人: MIYAMOTO Hajime
• 金额: $ 9.66万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11460127/
• 关键词: Ovary; Follicle; Granulosa cell; Mammal; Apoptosis; Cell-death receptor; Monoclonal antibody; Programmed cell death; アポトーシス受容体; デコイ受容体; HVJ-リポソーム; 遺伝子導入; ブタ; モノクロナール抗体

In mammalian ovaries, more than 99% of the follicles undergo the degenerative change known as atresia at varying stages of follicle development. our studies of follicular atresia have revealed the morphological and biochemical characteristics of atretic follicles. our recent findings have suggested that the degeneration of atretic follicles in mammalian ovaries can be explained, at least in part, by apoptotic death of granulosa and theca interna cells. we confirmed that apoptosis occurs in granulosa cells but not cumulus cells in the atretic graafian follicles from porcine ovaries. apoptosis is the result of many stimuli that act through many signal transduction pathways culminating in the activation of endonucleases. Apoptotic stimuli and intracellular signal ransduction pathways involved in granulosa cell apoptosis remain to be determined. Therefore, understanding of the events that trigger activation of the endonucleases is essential to elucidate the pathways upstream of apoptosis. we produced monoclonal antibodies that recognize cell-surface antigens (a novel cell-death receptor) of granulosa cells and induce apoptosis of cultured granulosa cells. Such antibodies, especially PFG-1 that induces apoptosis of cultured granulosa cells, are good probes for studying the intracellular pathways of granulosa cell apoptosis in porcine atretio follicles.

在哺乳动物卵巢中，超过99%的卵泡在卵泡发育的不同阶段经历称为闭锁的退行性变化。我们对卵泡闭锁的研究揭示了闭锁卵泡的形态学和生物化学特征。我们最近的发现表明，哺乳动物卵巢中闭锁卵泡的退化至少部分可以通过颗粒细胞和内膜细胞的凋亡来解释。我们证实，在猪卵巢闭锁的Graafian卵泡中，凋亡发生在颗粒细胞而不是卵丘细胞。细胞凋亡是许多刺激的结果，这些刺激通过许多信号转导途径起作用，最终导致核酸内切酶的激活。颗粒细胞凋亡中涉及的凋亡刺激和细胞内信号转导通路仍有待确定。因此，理解触发核酸内切酶激活的事件对于阐明细胞凋亡的上游途径是至关重要的。我们制备了识别颗粒细胞的细胞表面抗原（一种新的细胞死亡受体）并诱导培养的颗粒细胞凋亡的单克隆抗体。这些抗体，特别是PFG-1，诱导培养的颗粒细胞凋亡，是很好的探针，研究颗粒细胞凋亡的细胞内途径在猪闭锁卵泡。

项目成果

Noboru Manabe,Akira Myoumoto,Chiemi Tajima,Hajime Miyamoto et al.: "Immunochemical characteristics of a novel cell death receptor and a decoy receptor on granulosa cells of porcine ovarian follicles."Cytotechnology. 33・2. 189-201 (2000)

Noboru Manabe、Akira Myoumoto、Chiemi Tajima、Hajime Miyamoto 等：“猪卵巢卵泡颗粒细胞上的新型细胞死亡受体和诱饵受体的免疫化学特征”。《细胞技术》33・2。

Noboru Manabe, Hajime Miyamoto et al.: "In vivo noninvasive nuclear magnetic resonance (NMR) technique for assessment of teratogenicity induced by chemicals"Chemical Engineering. 47・1. 110-114 (2002)

Noboru Manabe、Hajime Miyamoto 等：“用于评估化学物质致畸性的体内无创核磁共振（NMR）技术”《化学工程》47・1（2002）。

Noboru Manabe et al.: "A novel in vivo NMR technique to assess halocarbon induced hepatic failure in mice"Water Science and Technology. 17・1. 32-38 (2001)

Noboru Manabe 等人：“一种评估卤化碳诱导的小鼠肝功能衰竭的新型体内 NMR 技术”，《水科学与技术》17・1（2001 年）。

Mizuho Nakayama, Noboru Manabe, Hajime Miyamoto et al.: "Histochemical studies for comparison of regulatory mechanisms of follicular development in mammalian ovaries. In : Miyamoto H, Manabe N (eds), Reproductive biotechnology : Reproductive biotechnology

Mizuho Nakayama、Noboru Manabe、Hajime Miyamoto 等人：“比较哺乳动物卵巢卵泡发育调节机制的组织化学研究。见：Miyamoto H、Manabe N（编辑），生殖生物技术：生殖生物技术

Namiko Kiso, Noboru Manabe, Hajime Miyamoto et al.: "Abnormal accumulation of luteal bodies in ovaries of the senescence accelerated mouse (SAM). In : Miyamoto H, Manabe N (eds), Reproductive biotechnology : Reproductive biotechnology update and its relat

Namiko Kiso、Noboru Manabe、Hajime Miyamoto 等人：“加速衰老小鼠 (SAM) 卵巢中黄体体的异常积累。见：Miyamoto H、Manabe N（编辑），生殖生物技术：生殖生物技术更新及其相关