Real-time assessment technique for reproductive toxicity of environmental disruptors by nuclear magnetic resonance microscopy

核磁共振显微镜实时评估环境干扰物生殖毒性技术

• 批准号: 14360167
• 负责人: MIYAMOTO Hajime
• 金额: $ 9.22万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14360167/
• 关键词: environmental disruptors; magnetic resonance imaging; 3D-morphoanalysis; mammal; embryo; orphan receptor; receptor nock out; real-time assessment; 外因性内分泌撹乱物質

Nuclear magnetic resonance (NMR) microscopy is a integrated magnetic resonance imaging technique with an enhanced spatial resolution due to the use of a high static magnetic field and high magnetic field gradients. NMR is a useful tool for non-invasive, real-time and continuous examination of tissue and organs at the histological level. We developed the application techniques of NMR microscopy to assessment of morphology in mammalian embryos using a developmental disorder model induced by oral administration of environmental disruptors (EDs). EDs induce widespread malformation in developing mammalian embryos. Two-dimensional and/or three-dimensional NMR. micro-images demonstrated defects in skeleton and soft tissue similarly to conventional assessments. Although further improvements are required for rapid dynamic imaging of living embryos, the present techniques will provide new approach as for multi-parametric assessment of embryo development under physiological and pathological conditions.

核磁共振（NMR）显微镜是一种集成的磁共振成像技术，由于使用高静磁场和高磁场梯度，因此具有增强的空间分辨率。核磁共振是一种有用的工具，用于在组织学水平上对组织和器官进行非侵入性、实时和连续的检查。我们开发了应用技术的核磁共振显微镜评估哺乳动物胚胎的形态，使用的发育障碍模型引起的口服环境干扰物（ED）。ED在发育中的哺乳动物胚胎中诱导广泛的畸形。二维和/或三维NMR。显微图像显示骨骼和软组织的缺陷，与常规评估相似。尽管活体胚胎的快速动态成像还需要进一步改进，但本技术将为生理和病理条件下胚胎发育的多参数评估提供新的途径。

项目成果

Satoshi Imanishi, Noboru Manabe, Takako Tanaka, Reika Okamoto, Maki Morita, Miki Sugimoto, Hajime Miyamoto: "mRNA of GPCR5B/Raig2, a novel orphan receptor, expresses in murine placentae"Journal of Reproduction and Devevelopment. 48・4. 627-632 (2002)

Satoshi Imanishi、Noboru Manabe、Takako Tanaka、Reika Okamoto、Maki Morita、Miki Sugimoto、Hajime Miyamoto：“新型孤儿受体 GPCR5B/Raig2 的 mRNA 在小鼠胎盘中表达”《生殖与发育杂志》48・4。 632（2002）

Noboru Manabe: "Molecular mechanism of follicle selection in mammalian ovaries"Journal of Laboratory Animals (Kansai). 23・1. 71-78 (2002)

真锅升：“哺乳动物卵巢中卵泡选择的分子机制”实验动物杂志（关西）23・1（2002）。

Toshikatsu Matsui, Noboru Manabe, Yasufumi Goto, Naoko Inoue, Shinsuke Nadagawa, Akihisa Maeda, Susumu Nishihara, Hajime Miyamoto: "Animal Frontier Science (Sato E, Miyamoto H, Manabe Noboru eds. ), Role of the mitochondria-dependent pathway in granulosa

Toshikatsu Matsui、Noboru Manabe、Yasufumi Goto、Naoko Inoue、Shinsuke Nadakawa、Akihisa Maeda、Susumu Nishihara、Hajime Miyamoto：“动物前沿科学（Sato E、Miyamoto H、Manabe Noboru eds。），粒细胞中线粒体依赖性途径的作用

Noboru Manabe, Yasuyoshi Azuma, Miki Sugimoto, Kei-Ichi Sezaki, Mineko Iwahori, Satoshi Imanishi, Hanako Nishizawa, Takako Tanaka, Maki Morita, Hajime Miyamoto: "Animal Frontier Science (Sato E, Miyamoto H, Manabe Noboru eds.), In vivo and real time estim

Noboru Manabe、Yasuyoshi Azuma、Miki Sugimoto、Kei-Ichi Sezaki、Mineko Iwahori、Satoshi Imanishi、Hanako Nishizawa、Takako Tanaka、Maki Morita、Hajime Miyamoto：“动物前沿科学（佐藤 E、宫本 H、Manabe Noboru 编辑），In

Naoko Inoue, Noboru Manabe, Toshikatsu Matsui, Shinsuke Nakagawa, Akihisa Maeda, Hajime Miyamoto: "Changes in expression of fas-dependent apoptotic signaling molecules in granulosa cells during folliclar atresia in pig ovaries"Biology of Reproduction. 67.

Naoko Inoue、Noboru Manabe、Toshikatsu Matsui、Shinsuke Nakakawa、Akihisa Maeda、Hajime Miyamoto：“猪卵巢卵泡闭锁期间颗粒细胞中 fas 依赖性凋亡信号分子表达的变化”生殖生物学。