好気性および嫌気性膜分離活性汚泥法における薬剤耐性遺伝子の動態に関する研究

好氧、厌氧膜分离活性污泥过程中耐药基因动态研究

• 批准号: 18J15071
• 负责人: 馬 緻宇
• 金额: $ 0.96万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2018
• 资助国家: 日本
• 起止时间: 2018-04-25 至 2020-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-18J15071/
• 关键词: antibiotic resistance; e.coli; wwtp effluent; lake biwa

The release of antibiotics into the environment can result in antimicrobial resistance (AMR) spread, which in turn can impede the effective prevention of infections caused by pathogens. β-lactams are the most widely used antibiotics, and bacterial resistance to these antibiotics is a severe threat that has been widely reported. Among the β-lactams, ampicillin is one kind of penicillin-based antibiotics which is used to treat a wide variety of bacterial infections. Veterinary use of ampicillin is estimated to 49000 kg per year in Japan. The objectives of this study are to clarify antibiotic resistance characteristics and genetic characterization in ampicillin resistant Escherichia coli (E. coli) isolated from WWTPs influent, effluent and the receiving aquatic environment (the Lake Biwa) in Japa results provided the genetic characterization and comparison of E. coli from WWTPs influent, effluent and Lake Biwa under antibiotic (ampicillin) stress. And the results suggest that the antibiotic selection in aquatic environment will affect the genetic composition of E. coli populations. Further research should discuss and compare the effects of different antibiotics (e.g. sulphonamide, macrolid) on the prevalence of resistant bacteria in aquatic environments.

抗生素释放到环境中会导致抗菌素耐药性（AMR）的传播，这反过来又会阻碍病原体引起的感染的有效预防。β-内酰胺类抗生素是使用最广泛的抗生素，细菌对这些抗生素的耐药性是一个严重的威胁，已被广泛报道。在β-内酰胺类抗生素中，氨苄青霉素是一种以青霉素为基础的抗生素，用于治疗各种细菌感染。在日本，氨苄青霉素的兽医使用量估计为每年49000 kg。本研究的目的是阐明氨苄青霉素耐药大肠埃希菌的耐药性特征和遗传特征。coli）的遗传特性进行了比较。大肠杆菌从污水处理厂进水，出水和琵琶湖抗生素（氨苄青霉素）的压力。结果表明，水环境中抗生素的选择会影响大肠杆菌的遗传组成。大肠杆菌种群。进一步的研究应讨论和比较不同抗生素（如磺胺类、大环内酯类）对水生环境中耐药细菌流行的影响。

项目成果

Impacts assessment of WWTPs effluent on antimicrobial resistance dissemination of receiving aquatic ecosystem in Japan by whole-genome sequencing approach

通过全基因组测序方法评估污水处理厂废水对日本接收水生生态系统抗菌药物耐药性传播的影响

• 发表时间: 2019
• 作者: Chih-Yu Ma;Masaru Ihara;Siyao Liu;Naoyuki Yamashita;Hiroaki Tanaka
• 通讯作者: Hiroaki Tanaka

Antimicrobial resistance profiles among Escherichia coli strains isolated from Lake Biwa and wastewater treatment plants effluent revealed by Whole-genome sequencing analysis

通过全基因组测序分析揭示从琵琶湖和废水处理厂废水中分离出的大肠杆菌菌株的抗菌谱

• 发表时间: 2019
• 作者: Chih-Yu Ma;Masaru Ihara;Siyao Liu;Yoshiki Okuno;Yu Zaizhi; Naoyuki Yamashita and Hiroaki Tanaka
• 通讯作者: Naoyuki Yamashita and Hiroaki Tanaka

Genetic characteristics of ampicillin-resistant E. coli in wastewater treatment plants influent, effluent, and Lake Biwa

废水处理厂进水、出水和琵琶湖中耐氨苄青霉素大肠杆菌的遗传特征

• 发表时间: 2019
• 作者: 馬緻宇;井原賢; 劉思瑶 ;田中宏明
• 通讯作者: 田中宏明

Enhancing the water desalination and electricity generation of a microbial desalination cell with a three-dimensional macroporous carbon nanotube-chitosan sponge anode

• DOI: 10.1016/j.scitotenv.2019.04.174
• 发表时间: 2019-07-20
• 期刊: SCIENCE OF THE TOTAL ENVIRONMENT
• 影响因子: 9.8
• 作者: Ma, Chih-Yu;Hou, Chia-Hung
• 通讯作者: Hou, Chia-Hung