Development of an odor discrimination sensor mimicked insect olfactory system

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In this study, we aimed to establish the methodology for developing a cell-based odorant sensor with high sensitivity and good selectivity based on insect odorant receptors. Using genetic engineering technique, we demonstrated that the Sf21 cell lines expressing insect odorant receptors along with a fluorescent calcium indicator protein can sensitively detect general odorants by increasing their fluorescent intensities. In addition, using microfabrication technique we fabricated a microfluidic chip with two independent channels for simultaneously supplying odorant stimuli, and demonstrated that we were able to measure fluorescent responses of two Sf21 cell lines that were arranged in parallel with each other on the chip. Taken together, we established the basic technology for developing an odorant sensor chip by integrating the Sf21 cell lines into the microfluidic chip.