Development of humanized sugar chain in silkworm targeting for production of next-generation medical glycoprotein

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In order to provide an ability to synthesize sugar chains close to the complex type of mammalian cells from high mannose type of silkworm, we tried to modify the sugar chain synthetic pathway of silkworm. The activity of targeting N-acetylglucosaminidase in both Bm 5 cell and silkworm was decreased to 60% compared to control by adopting RNA silencing technology. As a test molecule IgG was expressed, and analyzed sugar chains. The paucimannose sugar chain of IgG accounted for 70% to 80% of the total, which suggests that silencing effect of N-acetylglucosaminidase was not enough to modify sugar chain synthetic pathway of silkworm. These results indicate that N-acetylglucosaminiltransferase may need to modify mannose type to complex type in silkworm.