Development of a novel drug-delivery system by a bio-capsule that is built by primary intestinal epithelial cells.

通过由原代肠上皮细胞构建的生物胶囊开发新型药物递送系统。

• 批准号: 23659394
• 负责人: WATANABE Mamoru
• 金额: $ 2.33万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23659394/
• 关键词: 腸管上皮; バイオカプセル; 薬剤デリバリー; 炎症性腸疾患; 初代培養法

Through our studies aimed to establish novel methods to generate “bio-capsules” that are built by intestinal epithelial cells, we succeeded to identify a distinct culture condition, which allows us to produce “bio-capsules” in a highly efficient manner. In addition, we found that the capsules that were generated by the present method are consisted with high number of intestinal stem cells. Also, we confirmed that Rhodamine123, a substrate of P-glycoprotein, could easily accumulate within the inner space of the bio-capsule. These findings establish the basis for the efficient generation of drug-containing, intestinal epithelial cell-derived bio-capsules.

通过我们的研究旨在建立新的方法来产生由肠上皮细胞构建的“生物胶囊”，我们成功地确定了一种独特的培养条件，使我们能够以高效的方式生产“生物胶囊”。此外，我们发现通过本方法产生的胶囊由高数量的肠干细胞组成。此外，我们证实，罗丹明123，P-糖蛋白的底物，可以很容易地积累在生物胶囊的内部空间。这些发现为有效产生含有药物的肠上皮细胞衍生的生物胶囊奠定了基础。

项目成果

Regeneration of damaged colon epithelium by transplanted colon Lgr5+ stem cells maintained and expanded in vitro

通过体外维持和扩增的移植结肠 Lgr5 干细胞实现受损结肠上皮的再生

• 发表时间: 2012
• 作者: Yui S;Nakamura T;Nemoto Y;Mizutani T;Fukuda M;Nozaki K;Yamauchi Y;Mochiduki W;Zheng X;Nagaishi T;Okamoto R;Tsuchiya K;Watanabe M
• 通讯作者: Watanabe M

Real-time analysis of p-glycoprotein-mediated drug transport across primary intestinal epitherial cells three-dimensionally cultured in vitro

实时分析 p-糖蛋白介导的体外三维培养原代肠上皮细胞的药物转运

• 发表时间: 2012
• 作者: Mizutani T;Watanabe M et.al.
• 通讯作者: Watanabe M et.al.

Luminal CD4+T cells penetrate gut epithelial monolayers and egress from lamina propia to blood circulation

腔内 CD4 T 细胞穿透肠道上皮单层并从固有层进入血液循环

• 发表时间: 2011
• 作者: Nemoto Y;Watanabe M
• 通讯作者: Watanabe M

Regeneration of damaged colon epithelium by transplanted colon Lgr5+ stem cells maintained and expanded in vitro.

通过移植的结肠 Lgr5 干细胞在体外维持和扩增受损的结肠上皮的再生。

• 作者: Yui S;Watanabe M et.al.
• 通讯作者: Watanabe M et.al.

Real-time analysis of p-glycoprotein-mediated drug transport across primary intestinal epitherial cells three-dimensionallycultured in vitro

实时分析 p-糖蛋白介导的体外三维培养原代肠上皮细胞的药物转运

• 发表时间: 2012
• 作者: Mizutani T;Nakamura T;Morikawa R;Fukuda M;Mochizuki W;Yamauchi Y,Nozaki K;Yui S;Okamoto R;Tsuchiya K;Watanabe M
• 通讯作者: Watanabe M