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Dynamics of Eupyrene and Apyrene Sperm in the Spermatophore of the Swallowtail Butterfly and the Investment of Males

燕尾蝶精囊中丁芘和阿芘精子的动态及雄性的着精

基本信息

批准号：
10640612
负责人：
WATANABE Mamoru
金额：
$ 2.5万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

When the swallowtail butterflies, Papilio xuthus, are mated by the hand-pairing method, both types of sperm, eupyrene and apyrene sperm, are transferred from the male to the spermatheca via the spermatophore in the bursa copulatrix. This is demonstrated by two different kinds of experiments. The first set of experiments employed interrupted copulation, and the second set was examination of the sperm in the spermatophore and spermatheca after the termination of copulation. The sperm was transferred 30 min after the start of copulation. The eupyrene sperm was still in the bundle, the number of the bundles ranged from 9 to 108 (mean 42.7, n=27) . The bundles were gradually released after the completion of copulation, and then the free eupyrene spermatozoa remained in the spermatophore at least 2 h before migrating to the spermatheca. On the other hand, about 160,000 apyrene spermatozoa were transferred to the spermatophore and remained there for more than 1 h. 11,000 apyrene spermatozoa in the spermatheca were observed 12 h after the completion of copulation, but most of this type of sperm disappeared shortly thereafter. In contrast, the eupyrene sperm arrived in the spermatheca more than 1 day after the completion of copulation, and remained there at least one week. Therefore, these findings suggest that apyrene sperm migrate from the spermatophore to the spermatheca earlier than eupyrene sperm. Accordingly, if females mated multiply, the time difference might avoid the mixing of sperm. In addition, the predominance of sperm from the last mating session may occur not in the bursa copulatrix but in the spermatheca.

当燕尾蝶（Papilio xuthus）用手配对的方法进行交配时，两种类型的精子，正炔精子和非正炔精子，都通过囊交媾基质中的精子包囊从雄性转移到精囊中。两种不同的实验证明了这一点。第一组实验采用中断交配，第二组实验采用终止交配后精子包囊和精囊内精子的检测。精子在交配开始30分钟后被转移。真核精子仍在卵束中，卵束数为9 ~ 108个（平均42.7个，n=27）。交配完成后，精子束逐渐释放，游离的正生精子在精子包囊中至少停留2小时，然后迁移到精囊中。另一方面，约有16万个无性系精子被转移到精子包囊中，并在精子包囊中停留了1小时以上。在交配完成12小时后，在精子包囊中观察到1.1万个无性系精子，但这类精子大部分在交配完成后不久就消失了。相比之下，正核精子在交配完成后1天以上到达精子囊，并在那里至少停留一周。因此，这些研究结果表明，无倍体精子比真倍体精子更早地从精包迁移到精囊。因此，如果雌性交配繁殖，时间差可能会避免精子的混合。此外，最后一次交配的精子优势可能不是发生在交配囊的性交基质中，而是发生在精囊中。