Application! Of low temperature microplasma to the treatment and diagnosis for gastrointestinal disease

应用！

• 批准号: 13557046
• 负责人: WATANABE Mamoru
• 金额: $ 8.77万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13557046/
• 关键词: microplasma; low temperature plasma; endoscopy; drug; biological active substance; inflammation; cancer; 低音プラズマ; 生理活性物資

In this study, we have made attempts to develop new devices that can be employed through the channel of the gastrointestinal (GI) endoscopy and function as sources of low-pressure and high-density plasma, aiming at the clinical application of these microelectronic devices for delivering various chemical and/or bioactive compounds to the mucosal surface of the GI tract. One of the critical steps for the development of such device is narrowing its diameter, and, collaborating with a group of Keio University, we have successfully developed a flexible and multitask plasma processing device that is less than 1 mm in diameter. In addition, using very high frequency (VHF) power source that is recently developed, we have advanced our technology to be widely applicable to various materials. Furthermore, preliminary studies demonstrated that our device, designed to generate low temperature microplasma, showed advantages over other devices we examined with regard to the limitation of the mucosal surface damage. These devices would allow a wide variety of drugs and bioactive materials to be topically and efficiently delivered to the target molecules, such as bacterial components or the products of genes that are involved in inflammatory responses or cellular oncogenesis, and therefore, would serve as an innovative and powerful tool for diagnostic and therapeutic approaches to human diseases of the GI tract.

在这项研究中，我们尝试开发可通过胃肠道（GI）内窥镜通道使用并作为低压高密度等离子体源的新设备，旨在将这些微电子设备用于向胃肠道粘膜表面输送各种化学和/或生物活性化合物的临床应用。开发这种设备的关键步骤之一是缩小其直径，我们与庆应义塾大学的团队合作，成功开发了一种直径小于1毫米的灵活的多任务等离子体处理设备。此外，使用最近开发的甚高频（VHF）电源，我们的技术得到了进步，可广泛应用于各种材料。此外，初步研究表明，我们的设备旨在产生低温微等离子体，在限制粘膜表面损伤方面比我们检查的其他设备显示出优势。这些装置将允许多种药物和生物活性材料局部有效地递送到目标分子，例如参与炎症反应或细胞肿瘤发生的细菌成分或基因产物，因此，将成为人类胃肠道疾病诊断和治疗方法的创新和强大工具。

项目成果

Kanai T, Watanabe M, et al.: "IL-18 is a potent proliferative factor for intestinal mucosal lymphocytes in Crohn's disease"Gastroenterolog. 119. 1514-1523 (2000)

Kanai T、Watanabe M 等人：“IL-18 是克罗恩病肠粘膜淋巴细胞的有效增殖因子”Gastroenterolog。

Kanai, T, Watanabe, M, et al.: "Macrophage-derived IL-18 mediated intestinal inflammation in the murine model of Crohn's disease"Gastroenterology. 121. 875-888 (2001)

Kanai, T、Watanabe, M 等人：“克罗恩病小鼠模型中巨噬细胞衍生的 IL-18 介导的肠道炎症”胃肠病学。

Okamoto, R., Yajima, T., Yamazaki, M., Kanai, T., Mukai, M., Okamoto, S., Ikeda, Y., Hibi, T., Inazawa, J., Watanabe, M.: "Damaged epithelia regenerated by bone marrow-derived cells in the human gastrointestinal tract"Nature Med. 8. 1011-1017 (2002)

冈本 R.、矢岛 T.、山崎 M.、金井 T.、向井 M.、冈本 S.、池田 Y.、日比 T.、稻泽 J.、渡边 M.：

Totsuka, T., Kanai, T., liyama, R., Uraushihara, K., Yamazaki, M., Okamoto, R., Hibi, T., Tezuka, K., Azuma, M., Akiba, H., Yagita, H., Okumura, K., Watanabe, M.: "Ameliorating Effect of Anti-Inducible CpStimulator Monoclonal Antibody in a Murine Model of

户冢 T.、金井 T.、liyama, R.、浦石原 K.、山崎 M.、冈本 R.、日比 T.、手冢 K.、东 M.、秋叶 H.、

Totsuka T, Watanabe M, et al.: "Ameliorating Effect of Anti-Inducible Costirniilator Monoclonal Antibody in a Murine Model of Chronic Colitis"Gastroenterology. 124. 410-421 (2002)

Totsuka T、Watanabe M 等人：“抗诱导共刺激单克隆抗体在慢性结肠炎鼠模型中的改善作用”胃肠病学。