Understanding of the in vivo temporal patterns of insulin

据报道，血液胰岛素的时间模式对于生理稳态是重要的。我们揭示了使用培养细胞的胰岛素的时间模式的重要性，然而，在我们体内的重要性仍然未知。本研究作为一项“探索性研究”，建立了一个胰岛素直接注射刺激大鼠的实验系统。结果，我们可以在（i）通过颈静脉注射生长抑素以抑制内源性胰岛素释放，（ii）通过颈静脉注射葡萄糖以维持血糖水平和（iii）通过肠系膜静脉注射胰岛素的条件下刺激大鼠。

It is reported that temporal patterns of blood insulin are important for physiological homeostasis. We revealed that the importance of temporal patterns of insulin using cultured cells, however, the importance in our body remains unknown. In this proposal, as a "Challenging Exploratory Research", we established an experimental system to stimulate rats by a direct insulin injection. As results, we can stimulate rats under conditions of (i) a somatostatine injection via jugular vein to suppress the endogenous insulin release, (ii) a glucose injection via jugular vein to maintain blood glucose level and (iii) an insulin injection via mesenteric vein.