Development of a novel in vivo imaging system and its application to place cells

新型体内成像系统的开发及其在细胞定位中的应用

• 批准号: 24650209
• 负责人: WAKAZONO Yoshihiko
• 金额: $ 2.41万
• 依托单位: University of Miyazaki
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24650209/
• 关键词: in vivo イメージング; 自由行動; 海馬; 神経活動

In the present work, we tried to develop a novel in vivo imaging system, which can be equipped with a freely moving animal for a long period, to understand higher brain functions.We first made green fluorescence protein (GFP)-expressing lentivirus vector, and then injected it to mouse hippocampus CA1 region. A few weeks later, strong GFP signals were observed in the region of the acute slice under a fluorescence microscope. In addition, the lentivirus infection had no influence on electrophysiological properties, including long-term potentiation (LTP) expression, of GFP-expressed neurons. Next, we tried to observed fluorescence images of the similar slice via gradient index (GRIN) lens, however, the images were faint. GRIN lens is thought to play a key role in in vivo imaging system because it transfer the images from the inside of the brain to the outside. In the future, therefore, it is necessary to improve the system by increasing signal-to-noise ratio.

在本工作中，我们试图开发一种新型的体内成像系统，该系统可以与自由运动的动物长期配对，以了解高级脑功能。我们首先制作了表达绿色荧光蛋白(GFP)的慢病毒载体，然后将其注射到小鼠海马CA1区。几周后，在荧光显微镜下观察到急性切片区域有较强的GFP信号。此外，慢病毒感染对GFP表达的神经元的电生理特性，包括长时程增强(LTP)的表达没有影响。接下来，我们尝试通过梯度折射率(GRIN)透镜观察到类似切片的荧光图像，但图像很模糊。GRIN透镜被认为在活体成像系统中起着关键作用，因为它将图像从大脑内部传输到外部。因此，未来有必要通过提高信噪比来改进系统。

项目成果

Application of hippocampus CA1-selective gene transfer system for analyzing LTP expression mechanisms

应用海马CA1选择性基因转移系统分析LTP表达机制

• 发表时间: 2012
• 作者: 若園佳彦;蔦島譲治;スワチャンダソングメン;國武孝人;高宮考悟
• 通讯作者: 高宮考悟

An application of selective injection system into hippocampus CA1 under monitoring of theta oscillation for analyzing LTP expression

应用选择性注射系统在θ振荡监测下海马CA1区分析LTP表达

• 发表时间: 2013
• 作者: 若園佳彦;蔦島譲治;國武孝人;高宮考悟
• 通讯作者: 高宮考悟

Development and application examples of selective injection system into hippocampus CA1 via monitored theta oscillation

通过监测θ振荡选择性海马CA1注射系统的开发和应用实例

• 发表时间: 2014
• 作者: 若園佳彦;蔦島譲治;高宮考悟
• 通讯作者: 高宮考悟