Functional analyses of newborn neurons in CNS by Cereblon overexpression

过度表达Cereblon(CRBN)的斑马鱼胚胎表现出大脑增大。在这项研究中，我们通过以下方法研究了这些增加的神经元是否在动物的高级行为中发挥实际作用：1)使用钙传感器测量神经活动：(结果)在受精后6天，CRBN过表达的幼虫在静息和光刺激条件下受精后6天，端脑中的神经活动增加到正常的140%。2)通过光刺激和电击相结合的双向主动回避实验测量学习潜力：(结果)在本实验中，CRBN过表达的幼虫的学习能力高于正常幼虫。

Zebrafish embryos overexpressing Cereblon (CRBN) showed enlarged brain. They have excess number of neurons as well as glia cells with spatially normal pattern.In this study, we investigated whether those increased neurons actually function in higher behavior of the animal by the following approaches.1)Measurement of neural activity by the use of calcium sensor: (Results) Neural activities in the telencephalon were increased to 140% of normal ones in CRBN-overexpressed larvae at 6 days after fertilization both in resting condition and in light-stimulated condition.2)Measurement of learning potential by the 2-directional active avoidance assay using combination of light stimulation and electrical shock: (Results) In this experiment, CRBN-overexpressed larvae showed higher score than normal ones.