Functional analyses of newborn neurons in CNS by Cereblon overexpression

通过 Cereblon 过表达对中枢神经系统新生神经元进行功能分析

• 批准号: 24650231
• 负责人: ANDO Hideki
• 金额: $ 2.5万
• 依托单位: Tokyo Medical University (2013)Tokyo Institute of Technology (2012)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24650231/
• 关键词: ゼブラフィッシュ; 脳機能; Cereblon; 脳再生

Zebrafish embryos overexpressing Cereblon (CRBN) showed enlarged brain. They have excess number of neurons as well as glia cells with spatially normal pattern.In this study, we investigated whether those increased neurons actually function in higher behavior of the animal by the following approaches.1)Measurement of neural activity by the use of calcium sensor: (Results) Neural activities in the telencephalon were increased to 140% of normal ones in CRBN-overexpressed larvae at 6 days after fertilization both in resting condition and in light-stimulated condition.2)Measurement of learning potential by the 2-directional active avoidance assay using combination of light stimulation and electrical shock: (Results) In this experiment, CRBN-overexpressed larvae showed higher score than normal ones.

过度表达Cereblon（CRBN）的斑马鱼胚胎表现出大脑扩大。在本研究中，我们通过以下方法研究了这些增加的神经元是否在动物的高级行为中实际起作用。1）通过使用钙传感器测量神经活动：（结果）CRBN端脑神经元活动增强，为正常的140%，CRBN端脑神经元活动增强，为正常的140%，CRBN端脑神经元活动增强。2）通过使用光刺激和电击的组合的双向主动回避测定来测量学习潜力：（结果）在本实验中，CRBN过表达的幼虫表现出比正常幼虫更高的评分。

项目成果

Vesnarinone Suppresses TNF α mRNA Expression by Inhibiting Valosin-containing Protein

Vesnarinone 通过抑制 Valosin 含蛋白来抑制 TNF α mRNA 表达

• DOI: 10.1124/mol.112.081935
• 发表时间: 2013
• 期刊: Mol Pharmacol
• 影响因子: 3.6
• 作者: Hotta K;Nashimoto A;Yasumura E;Suzuki M;Azuma M;Iizumi Y;Shima D;Nabeshima R;Hiramoto M;Okada A;Sakata-Sogawa K;Tokunaga M;Ito T;Ando H;Sakamoto S;Kabe Y;Aizawa S;Imai T;Yamaguchi Y;Watanabe H;Handa H
• 通讯作者: Handa H

Cereblon, a new regulator of stem cell proliferation in zebrafish central nervous system

Cereblon，斑马鱼中枢神经系统干细胞增殖的新调节剂

• 发表时间: 2013
• 作者: Hideki Ando;Takumi Ito;Hiroshi Handa
• 通讯作者: Hiroshi Handa