Functional role of adrenergic receptor in skeletal muscle plasticity

肾上腺素能受体在骨骼肌可塑性中的功能作用

• 批准号: 24650409
• 负责人: KAZUHIKO Imaizumi
• 金额: $ 2.41万
• 依托单位: Waseda University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24650409/
• 关键词: 骨格筋の可塑性; アドレナリン受容体; アドレナリン作動薬; クレンブテロール; 骨格筋の組織化学; II 型筋線維; I 型筋線維; 骨格筋萎縮の抑制; β2‐作動薬; 骨格筋萎縮; 細胞内情報伝達; 骨格筋肥大; β2-アドレナリン受容体; β2-アゴニスト; 骨格筋; 筋肥大; マウス

We investigated whether daily administration of clenbuterol(CLE;1mg/kg BW per day) prevented casted-immobilization(IMM)-induced atrophy of skeleta muscle fibers. Adult male Sprague-Dawley rats were assigned to control(CON), CLE, IMM, and IMM+CLE groups, and were maintained for 9 days. The extensor digitorum longus(EDL) and soleus(SOL) muscles were isolated and analyzed histochemically after ATPase staining. EDL and SOL muscle weights in the IMM group were lower than those in the CON group. Quantitative analysis of cross-sectional areas revealed that EDL muscle atrophy was limited to type II fibers; SOL muscle atrophy involved type I and type II fibers. In the IMM+CLE group, IMM-induced muscle atrophy was attenuated in the EDL, but not in the SOL muscle. Moreover, the attenuating effect of CLE was observed in type II fibers. These results suggest that CLE attenuated IMM-induced atrophy of type II fibers in the fast-twitch EDL muscle but not in the slow-twitch SOL muscle.

我们调查了克伦特罗(Clenbuterol，每天1 mg/kg体重)是否能预防铸型制动(IMM)引起的骨骼肌纤维萎缩。将成年雄性SD大鼠分为对照组(CON)、CLE组、IMM组和IMM+CLE组，维持9天。分离指长伸肌(EDL)和比目鱼肌(SOL)，ATPase染色后进行组织化学分析。IMM组EDL和SOL肌重均低于CON组。横截面积的定量分析显示，EDL肌萎缩仅限于II型纤维；SOL肌萎缩涉及I型和II型纤维。在IMM+CLE组，IMM诱导的肌萎缩在EDL减轻，但在SOL肌没有减轻。此外，在II型纤维中观察到了CLE的衰减作用。这些结果表明，CLE可减轻IMM引起的快抽动EDL肌中II型纤维的萎缩，但不能减轻慢抽动SOL肌中II型纤维的萎缩。

项目成果

Spr inger JFunctional roles ofβ_2-adrenergic receptors in skeletal muscle hypertrophy and atrophy. In : Sports Sciences for the Promotion of an Active Life. Vol. II. Physical Activity, Exercise. Sedentary Behaviour and Health Promotion (Ed. by Kanosue, et

Springer J《骨骼肌肥大和萎缩中的β_2-肾上腺素能受体的功能》，《促进积极生活的运动科学》，《久坐行为和健康促进》。

• 发表时间: 2014
• 作者: Shirato K;Sato S;Sato M;Hashizume Y;Tachiyashiki K and Imaizumi K
• 通讯作者: Tachiyashiki K and Imaizumi K

β_2-gonist clenbuterol suppresses bacterial phagocytosis of splenic macrophages expressing high levels of macrophage receptor with collageneous structure

β_2-激动剂克伦特罗抑制表达高水平具有胶原结构的巨噬细胞受体的脾巨噬细胞的细菌吞噬作用

• 发表时间: 2013
• 期刊: Biological & Pharmaceutical Bulltin
• 作者: Shirato K;Sato S;Sato M;Hashizume Y;Tachiyashiki K and Imaizumi
• 通讯作者: Tachiyashiki K and Imaizumi

Functional roles of β2-adrenergic receptors in skeletal muscle hypertrophy and atrophy. In : Sports Sciences for the Promotion of an Active Life, II. Physical Acitivity, Exercise, Sedentary Behaviour and Health Promotion

β2-肾上腺素能受体在骨骼肌肥大和萎缩中的功能作用：促进积极生活的运动科学，II。

• 发表时间: 2014
• 作者: Sato S;Shirato K;Mitsuhashi R;Suzuki K;Tachiyashiki K; Imaizumi K
• 通讯作者: Imaizumi K

Effects of β2-agonist administration on bacterial macrophage phagocyososis by macrophages in mice. In : Sports Sciences for the Promotion of an Active Life, II. Physical Acitivity, Exercise, Sedentary Behaviour and Health Promotion

β2-激动剂对小鼠巨噬细胞吞噬作用的影响：促进积极生活的运动科学，II。

• 发表时间: 2014
• 作者: Shirato K;Sato S;Sato M;Hashizume Y;Tachiyashiki K;Imaizumi K
• 通讯作者: Imaizumi K

Effects of exercise on the hexosamine biosynthetic pathway and glycosylation

运动对己糖胺生物合成途径和糖基化的影响

• 发表时间: 2012
• 期刊: J. Phys. Fit. Sports Med
• 作者: Shirato;K.;Kizaki;T.;Ohno;H.;& Imaizumi;K.
• 通讯作者: K.