Establishment of a novel method to identify the hydroxymethylcytosine in single base resolution.

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DNA methylation is one of the important modifications in epigenetic regulation of mammalian genome system. Recently, the oxidation reaction of methylcytosine to hydroxymethylcytosine is discovered. Thus, it is important to establish a method to identify the hydroxymethylcytosine of the genome in single base resolution. Here I provide a novel method to identify the methylcytosine and the hydroxymethylcytosine with high accuracy (more than 95%) using DNMT1 enzyme.