development of preparation of modified tRNA using protein
development of preparation of modified tRNA using protein
批准号:
24655145
负责人:
TANAKA Yoshikazu
金额:
$2.58万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2012
资助国家:
日本
项目状态:
已结题
起止时间:
2012-04-01 至 2014-03-31
中文摘要
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英文摘要
In this study, I tried to establish conventional method to prepare highly pure modified tRNA from E. coli cell using tRNA-protein complex spontaneously formed in the cells. First, I established preparation system for tRNA(Met) using E. coli tRNA(Met) acetyltransferase. For this purpose, I selected K205A mutant, which has high affinity for tRNA. Moreover, crystals of the purified tRNA(Met) were obtained. The crystals diffracted about 6-angstrom resolution at synchrotron radiation facility. Furthermore, co-expression system of selenium introducing enzyme with tRNA(Gln), tRNA(Glu), and tRNA(Lys) in E. coli were constructed, and pure tRNAs were prepared from the tRNA-protein complex spontaneously formed in the E. coli cell.
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DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
Structural and genomic DNA analysis of a putative transcription factor SCO5550 from Streptomyces coelicolor A3 (2) : Regulating the expression of gene sco5551 as a transcriptional activator with a novel diner shape
天蓝色链霉菌 A3 的假定转录因子 SCO5550 的结构和基因组 DNA 分析 (2):调节 sco5551 基因的表达,作为具有新型晚餐形状的转录激活剂
DOI:
10.1016/j.bbrc.2013.04.017
发表时间:
2013
期刊:
Biochemical and Biophysical Research Communications
影响因子:
3.1
作者:
[Takeshi Hayashi, Yoshikazu Tanaka, Naoki Sakai, Nobuhisa Watanabe, Tomohiro Tamura, Isao Tanaka, Min Yao]
通讯作者:
Min Yao
The binding mechanism of eIF2β with its partner proteins, eIF5 and eIF2Bε, Biochem
eIF2β与其伴侣蛋白eIF5和eIF2Bε的结合机制,Biochem
DOI:
10.1016/j.bbrc.2012.05.155
发表时间:
2012
期刊:
Biophys. Res. Commun
影响因子:
--
作者:
[Gai Z, Kitagawa Y, Tanaka Y, Shimizu N, Komoda K, Tanaka I, Yao M]
通讯作者:
Yao M
Application of Photoactive Yellow Protein as a Photoresponsive Module for Controlling Hemolytic Activity of Staphylococcal α-Hemolysin
光活性黄色蛋白作为光响应模块在控制葡萄球菌α-溶血素溶血活性中的应用
DOI:
10.1039/c2cc18118e
发表时间:
2012
期刊:
Chemical Communications
影响因子:
4.9
作者:
[M. Ui, et al.]
通讯作者:
et al.
Crystal structure analysis of a DING protein, International life-science symposium for young scientists
DING 蛋白的晶体结构分析,国际青年科学家生命科学研讨会
DOI:
--
发表时间:
2014
期刊:
影响因子:
--
作者:
[Zuoqi Gai, Akiyoshi Nakamura, Yoshikazu Tanaka, Nagisa Hirano, Isao Tanaka, and Min Yao]
通讯作者:
and Min Yao
共 9 条
Study on practical energy harvesting using FPED and engine vibration
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批准号:15K06685
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项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$3.08万
-
财政年份:2015
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负责人:TANAKA Yoshikazu
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依托单位:
Study on environmental friendly submerged wave power generation system using FPED
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批准号:24760729
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项目类别:Grant-in-Aid for Young Scientists (B)
-
资助金额:$2.83万
-
财政年份:2012
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负责人:TANAKA Yoshikazu
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依托单位:
Functional analysis of cyclophilins on feline infectious peritonitis virus replication
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批准号:22580343
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$3.0万
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财政年份:2010
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负责人:TANAKA Yoshikazu
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依托单位:
Structural and functional analysis of Uro-adherence factor A(UafA) from Staphylococcus saprophyticus
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批准号:21770101
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项目类别:Grant-in-Aid for Young Scientists (B)
-
资助金额:$2.83万
-
财政年份:2009
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负责人:TANAKA Yoshikazu
-
依托单位:
Research and Development of Resonant Soft-X-ray Diffraction Under High Magnetic Field
-
批准号:21244049
-
项目类别:Grant-in-Aid for Scientific Research (A)
-
资助金额:$31.28万
-
财政年份:2009
-
负责人:TANAKA Yoshikazu
-
依托单位:
Analysis of regulation on feline infectious peritonitis virus replication by RNA interference
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批准号:19580351
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项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$3.08万
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财政年份:2007
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负责人:TANAKA Yoshikazu
-
依托单位:
Structural and functional analysis of iron uptake related cell-wall anchored protein Isd from Staphylococcus aureus
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批准号:19770076
-
项目类别:Grant-in-Aid for Young Scientists (B)
-
资助金额:$2.41万
-
财政年份:2007
-
负责人:TANAKA Yoshikazu
-
依托单位:
Analysis for selective ubiquitination of host immune proteins by Bovine Herpes virus type4 proteins
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批准号:16580246
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.37万
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财政年份:2004
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负责人:TANAKA Yoshikazu
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依托单位:
Detection of volcano-magnetic changes by aero-magnetic survey
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批准号:15403007
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$8.26万
-
财政年份:2003
-
负责人:TANAKA Yoshikazu
-
依托单位:
Molecular mechanisms of cataract and glaucoma induced by mutations in the Na-HCO3 cotransporter
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批准号:13671826
-
项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.3万
-
财政年份:2001
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负责人:TANAKA Yoshikazu
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依托单位:
Functional analysis of PML protein in the ubiquitin system
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批准号:13670156
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$0.77万
-
财政年份:2001
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负责人:TANAKA Yoshikazu
-
依托单位:
Crustal Electric Structure in the East-china Sea Observed by Submarine Cable
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批准号:11640416
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.3万
-
财政年份:1999
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负责人:TANAKA Yoshikazu
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依托单位:
Thermal state observed by declination magnetometers on Aso volcano
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批准号:08640532
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项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$1.22万
-
财政年份:1996
-
负责人:TANAKA Yoshikazu
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依托单位:
The Electrical Structure of Volcano Aso.
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批准号:61540290
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.22万
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财政年份:1986
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负责人:TANAKA Yoshikazu
-
依托单位:
海外基金