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development of preparation of modified tRNA using protein

development of preparation of modified tRNA using protein
利用蛋白质制备修饰tRNA的进展
批准号:
24655145
负责人:
TANAKA Yoshikazu
金额:
$2.58万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2012
资助国家:
日本
项目状态:
已结题
起止时间:
2012-04-01 至 2014-03-31

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中文摘要
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英文摘要
In this study, I tried to establish conventional method to prepare highly pure modified tRNA from E. coli cell using tRNA-protein complex spontaneously formed in the cells. First, I established preparation system for tRNA(Met) using E. coli tRNA(Met) acetyltransferase. For this purpose, I selected K205A mutant, which has high affinity for tRNA. Moreover, crystals of the purified tRNA(Met) were obtained. The crystals diffracted about 6-angstrom resolution at synchrotron radiation facility. Furthermore, co-expression system of selenium introducing enzyme with tRNA(Gln), tRNA(Glu), and tRNA(Lys) in E. coli were constructed, and pure tRNAs were prepared from the tRNA-protein complex spontaneously formed in the E. coli cell.
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北海道大学 X線構造生物学研究室 ホームページ
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DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
Structural and genomic DNA analysis of a putative transcription factor SCO5550 from Streptomyces coelicolor A3 (2) : Regulating the expression of gene sco5551 as a transcriptional activator with a novel diner shape
天蓝色链霉菌 A3 的假定转录因子 SCO5550 的结构和基因组 DNA 分析 (2):调节 sco5551 基因的表达,作为具有新型晚餐形状的转录激活剂
DOI: 10.1016/j.bbrc.2013.04.017
发表时间: 2013
期刊: Biochemical and Biophysical Research Communications
影响因子: 3.1
作者: [Takeshi Hayashi, Yoshikazu Tanaka, Naoki Sakai, Nobuhisa Watanabe, Tomohiro Tamura, Isao Tanaka, Min Yao]
通讯作者: Min Yao
The binding mechanism of eIF2β with its partner proteins, eIF5 and eIF2Bε, Biochem
eIF2β与其伴侣蛋白eIF5和eIF2Bε的结合机制,Biochem
DOI: 10.1016/j.bbrc.2012.05.155
发表时间: 2012
期刊: Biophys. Res. Commun
影响因子: --
作者: [Gai Z, Kitagawa Y, Tanaka Y, Shimizu N, Komoda K, Tanaka I, Yao M]
通讯作者: Yao M
Application of Photoactive Yellow Protein as a Photoresponsive Module for Controlling Hemolytic Activity of Staphylococcal α-Hemolysin
光活性黄色蛋白作为光响应模块在控制葡萄球菌α-溶血素溶血活性中的应用
DOI: 10.1039/c2cc18118e
发表时间: 2012
期刊: Chemical Communications
影响因子: 4.9
作者: [M. Ui, et al.]
通讯作者: et al.
9
    Study on practical energy harvesting using FPED and engine vibration
    • 批准号:
      15K06685
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $3.08万
    • 财政年份:
      2015
    • 负责人:
      TANAKA Yoshikazu
    • 依托单位:
    Study on environmental friendly submerged wave power generation system using FPED
    • 批准号:
      24760729
    • 项目类别:
      Grant-in-Aid for Young Scientists (B)
    • 资助金额:
      $2.83万
    • 财政年份:
      2012
    • 负责人:
      TANAKA Yoshikazu
    • 依托单位:
    Functional analysis of cyclophilins on feline infectious peritonitis virus replication
    Structural and functional analysis of Uro-adherence factor A(UafA) from Staphylococcus saprophyticus
    • 批准号:
      21770101
    • 项目类别:
      Grant-in-Aid for Young Scientists (B)
    • 资助金额:
      $2.83万
    • 财政年份:
      2009
    • 负责人:
      TANAKA Yoshikazu
    • 依托单位:
    海外基金