Breakthrough of long-term cultivating technologies for chemoautotrophic invertebrates

我们开发了一种提供硫化氢的饲养池系统，并培养了内共生生物和寄主无脊椎动物，包括Calyptogena spp.和Bathymodiolus sepdierum。Calyptogena在培养皿中存活43天。此外，九色芽孢杆菌存活时间超过14个月。利用荧光原位杂交技术，在培养3个月和14个月的个体鳃组织中观察到了九色虫的共生体。根据稳定同位素示踪实验，在H2S存在下，观察到培养3个月和14个月的个体的鳃和足组织从无机碳（碳酸氢盐）中吸收13C。

We developed a rearing tank system with supplying hydrogen sulfide and cultivated endosymbiont and the host invertebrates including Calyptogena spp. and Bathymodiolus septemdierum. Calyptogena spp. survived for 43 days in the tank. Furthermore, B. septemdierum survived for more than 14 months. The symbionts of B. septemdierum were observed in the gill tissues of 3 and 14 month-cultivated individuals based on fluorescence in situ hybridization analysis. According to a tracer experiment using stable isotope, 13C uptake in gill and foot tissues of 3 and 14 month-cultivated individuals from inorganic carbon, bicarbonate, observed under the presence of H2S.