Efficient production of novel biosurfactant by yeast isolated from deep sea

本文对湖北假酵母菌SY62产生的生物表面活性物质进行了详细的结构测定，该菌是从佐治亚湾海拔1156米处的大豆胞囊线虫(深海冷渗蚌，Shirouri-gai)中新分离的。核磁共振波谱结果表明，主要产物MEL-C是一种很有前途的BS。根据表面张力测定，新型MEL-C的临界胶束浓度(1.1×10-5M)大于结合C10和C12酸的传统MEL-C(9.1×10-6M)。我们证明了在改进的培养基中通过补料分批培养可以高效地生产MEL。一周后，MELS终浓度达129±8.2g/L。

We describe detailed structure determination of biosurfactant produced by Pseudozyma hubeiensis SY62, which was newly isolated from Calyptogena soyoae (deep-sea cold-seep clam, "Shirouri-gai") at 1,156 m in Sagami bay. Results of nuclear magnetic resonance spectroscopies indicated the major product, namely MEL-C, as a promising BS. According to surface tension determination, the novel MEL-C showed larger critical micelle concentration (1.1×10-5 M) than conventional MEL-C which bound C10 and C12 acids (9.1×10-6 M). We demonstrated a highly efficient production of MELs in the improved medium by fed-batch cultivation. The final concentration of MELs reached 129±8.2 g/L for one week.