Proteomic identification of dephosphorylated proteins by phosphatase protein

磷酸酶蛋白对去磷酸化蛋白的蛋白质组学鉴定

• 批准号: 24657033
• 负责人: FUKAO YOICHIRO
• 金额: $ 2.58万
• 依托单位: Nara Institute of Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24657033/
• 关键词: プロテインホスファターゼ; 脱リン酸化; リン酸化ペプチド精製; HPLC; ゼニゴケ; イネ培養細胞; PP2C; ホスファターゼ; リン酸化; ペプチド; タンパク質; プロテインフォスファターゼ; リン酸化ペプチド

Protein phosphorylation and dephosphorylation by protein kinases and phosphatases regulate many biological events in plants. To identify as many phosphoproteins as possible, several purification methods of phosphopeptides have been established. Here, we used two kind of phosphopeptides purification columns (PhosTio and PolyMac) and each method allowed identifying 771 and 641 phosphopeptides in rice cultured cells. Also, 913 and 802 phosphopeptides were identified in rice cultured cells treated with rice blast fungus. These results indicate that phosphorylation of several proteins are induced responded to pathogen attack and may have a role in disease resistances.

蛋白质磷酸化和去磷酸化通过蛋白激酶和磷酸酶调节植物中的许多生物学事件。为了鉴定尽可能多的磷蛋白，已经建立了几种磷酸肽的纯化方法。在这里，我们使用了两种磷酸肽纯化柱（PhosTio和PolyMac），每种方法都可以识别水稻培养细胞中的771和641磷酸肽。此外，在稻瘟病菌处理的水稻培养细胞中鉴定出913和802磷酸肽。这些结果表明，几种蛋白质的磷酸化被诱导响应病原体的攻击，并可能在抗病性的作用。