Examination of the function of insect male genitalia using a fine fluorescent beads

使用细荧光珠检查昆虫雄性生殖器的功能

• 批准号: 24657063
• 负责人: HAYASHI Fumio
• 金额: $ 2.5万
• 依托单位: Tokyo Metropolitan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24657063/
• 关键词: 交尾器; 昆虫; 配偶行動; 精子; 進化; 交尾行動; 交尾器の進化

Male genitalia of insects are complex in their shape including several independent parts. However, the function of each part of male genitalia remains mostly unknown. In this project, the attachment sites of male and female genitalia were examined using a fine fluorescent beads. These fluorescent beads were attached to the each part of male genitalia, and after copulation with the female we detected the beads on the female abdominal tips under the binocular scope. In mantids, we found that the asymmetric male genitalia functions in grasping the female ovipositor before spermatophore transfer. Thus, this method may be used for examining the insect male genital function.

昆虫的雄性生殖器形状复杂，包括几个独立的部分。然而，男性生殖器各部分的功能仍然大多未知。在这个项目中，使用细荧光珠检查男性和女性生殖器的附着部位。这些荧光珠附着在雄性生殖器的各个部位，与雌性交配后，我们在双目镜下检测到雌性腹部尖端的荧光珠。在螳螂科动物中，我们发现不对称的雄性生殖器的功能是在精囊转移之前抓住雌性产卵器。因此，该方法可用于检查昆虫雄性生殖功能。

项目成果

カマキリ類のオスの交尾器の左右非対称性とその特異な交尾行動

雄性螳螂交配器官左右不对称及其独特的交配行为

• 发表时间: 2014
• 作者: 鈴木航;指導教員:林文男
• 通讯作者: 指導教員:林文男