Examination of the function of insect male genitalia using a fine fluorescent beads

使用细荧光珠检查昆虫雄性生殖器的功能

• 批准号: 24657063
• 负责人: HAYASHI Fumio
• 金额: $ 2.5万
• 依托单位: Tokyo Metropolitan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24657063/
• 关键词: 交尾器; 昆虫; 配偶行動; 精子; 進化; 交尾行動; 交尾器の進化

Male genitalia of insects are complex in their shape including several independent parts. However, the function of each part of male genitalia remains mostly unknown. In this project, the attachment sites of male and female genitalia were examined using a fine fluorescent beads. These fluorescent beads were attached to the each part of male genitalia, and after copulation with the female we detected the beads on the female abdominal tips under the binocular scope. In mantids, we found that the asymmetric male genitalia functions in grasping the female ovipositor before spermatophore transfer. Thus, this method may be used for examining the insect male genital function.

昆虫的雄性生殖器是由多个独立的部分组成的，形状复杂。然而，男性生殖器的每个部分的功能仍然是未知的。在这个项目中，男性和女性生殖器的附着部位进行了检查，使用精细的荧光珠。将这些荧光珠附着在雄性生殖器的各个部位，与雌性交配后，在双目镜下检测雌性腹部尖端的荧光珠。在螳螂中，我们发现雄性不对称外生殖器的功能是在精荚转移前抓住雌性产卵器。因此，该方法可用于昆虫雄性生殖功能的检测。

项目成果

カマキリ類のオスの交尾器の左右非対称性とその特異な交尾行動

雄性螳螂交配器官左右不对称及其独特的交配行为

• 发表时间: 2014
• 作者: 鈴木航;指導教員:林文男
• 通讯作者: 指導教員:林文男