Mechanisms of sperm competition revealed by sperm viability assessment using live/dead dual fluorescence

使用活/死双荧光评估精子活力揭示精子竞争机制

• 批准号: 15570019
• 负责人: HAYASHI Fumio
• 金额: $ 2.3万
• 依托单位: Tokyo Metropolitan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15570019/
• 关键词: insect; sperm; mating behavior; genitalia; life history

Female damselflies in the family Calopterygidae have two sperm-storage organs, a spherical bursa copulatrix and a tubular spermatheca; males possess a peculiar aedeagus with a recurved head, with which to remove bursal sperm, and lateral spiny processes to remove spermathecal sperm. The lateral processes, however, differ among species or populations in terms of their width relative to the spermathecal duct; the narrower processes are physically able to access spermathecal sperm, while the wider ones are not. In this study, sperm-storage patterns and aedeagal structures were compared between two calopterygid species with different spermathecal structures, Calopteryx cornelia and Mnais pruinosa, with respect not only to sperm quantity (number) but also to sperm quality (viability), by using a recently developed method based on live/dead dual fluorescence. Calopteryx cornelia is a typical spermathecal sperm remover. In this species, viability was similar between bursal and spermathecal sperm. On the other hand, in M. pruinosa the spermatheca was much smaller than the bursa and often contained no sperm. Even when the spermatheca of this species did contain sperm, a high percentage was dead. While the spermatheca of M. pruinosa has such atrophic tendencies, males, have developed long and spiny lateral processes similar to those of C. cornelia. This suggests functions other than spermathecal sperm removal. The lateral processes possibly function as stoppers or guides for manipulating the aedeagal head to remove the sperm mass from the bursa.

豆娘科的雌性豆娘有两个精子储存器官，一个球形的交配囊和一个管状的受精囊。雄性拥有一种奇特的阳茎，头部向后弯曲，可以用来去除法氏囊精子，而侧面的刺状突起可以用来去除受精囊精子。然而，不同物种或种群的侧突相对于受精囊的宽度有所不同。较窄的突起在物理上能够接触到受精囊的精子，而较宽的突起则不能。在这项研究中，通过使用最近开发的基于活/死双荧光的方法，比较了具有不同受精囊结构的两种 Calopterygid 物种（Calopteryx cornelia 和 Mnais pruinosa）的精子储存模式和腺结构，不仅包括精子数量（数量），还包括精子质量（活力）。 Calopteryx cornelia 是一种典型的受精囊精子去除器。在该物种中，法氏囊和受精囊精子的活力相似。另一方面，M. pruinosa 的受精囊比法氏囊小得多，并且通常不含精子。即使该物种的受精囊确实含有精子，仍有很高比例死亡。虽然 M. pruinosa 的受精囊有萎缩的倾向，但雄性却发育出与 C. cornelia 相似的长而多刺的侧突。这表明除受精囊精子去除之外的其他功能。侧突可能起到止动件或引导件的作用，用于操纵阳头以从法氏囊中取出精子团。

项目成果

Distribution patterns of two damselfly species. Mnais costalis and M. strigata, in the Boso Peninsula, Chiba Prefecture

两种豆娘的分布格局。

• 发表时间: 2004
• 期刊: Tombo, (In Japanese) 47
• 作者: Futahashi;R.;Hayashi;F.
• 通讯作者: F.

日本産カワトンボ属の分類的,生態的諸問題への新しいアプローチ(1)総論

日本蜻蜓目分类学和生态问题的新方法（一）一般讨论

• 发表时间: 2004
• 期刊: Aeschna 41
• 作者: 林 文男;土畑重人;二橋 亮
• 通讯作者: 二橋 亮

日本産カワトンボ属の分類的,生態的諸問題への新しいアプローチ(2)資料

日本蜻蜓分类学和生态问题的新方法（2）材料

• 发表时间: 2005
• 期刊: Aeschna 42(印刷中)
• 作者: 林 文男;土畑重人;二橋 亮
• 通讯作者: 二橋 亮

Functional association between female sperm storage organs and male sperm removal organs in calopterygid damselflies

• DOI: 10.1111/j.1479-8298.2005.00123.x
• 发表时间: 2005-09-01
• 期刊: ENTOMOLOGICAL SCIENCE
• 影响因子: 0.9
• 作者: Hayashi, F;Tsuchiya, K
• 通讯作者: Tsuchiya, K

A new approach to resolve the taxonomic and ecological problems of Japanese Mnais damselflies (Odonata: calopterygidae)

解决日本豆娘分类学和生态问题的新方法（蜻蜓目：calopterygidae）

• 发表时间: 2005
• 期刊: (2) References and examined specimens, Aeschna, (In Japanese) 42 (in press)
• 作者: Hayashi;F.;Dobata;S.;Futahashi;R.
• 通讯作者: R.