Mechanisms of sperm competition revealed by sperm viability assessment using live/dead dual fluorescence

使用活/死双荧光评估精子活力揭示精子竞争机制

• 批准号: 15570019
• 负责人: HAYASHI Fumio
• 金额: $ 2.3万
• 依托单位: Tokyo Metropolitan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15570019/
• 关键词: insect; sperm; mating behavior; genitalia; life history

Female damselflies in the family Calopterygidae have two sperm-storage organs, a spherical bursa copulatrix and a tubular spermatheca; males possess a peculiar aedeagus with a recurved head, with which to remove bursal sperm, and lateral spiny processes to remove spermathecal sperm. The lateral processes, however, differ among species or populations in terms of their width relative to the spermathecal duct; the narrower processes are physically able to access spermathecal sperm, while the wider ones are not. In this study, sperm-storage patterns and aedeagal structures were compared between two calopterygid species with different spermathecal structures, Calopteryx cornelia and Mnais pruinosa, with respect not only to sperm quantity (number) but also to sperm quality (viability), by using a recently developed method based on live/dead dual fluorescence. Calopteryx cornelia is a typical spermathecal sperm remover. In this species, viability was similar between bursal and spermathecal sperm. On the other hand, in M. pruinosa the spermatheca was much smaller than the bursa and often contained no sperm. Even when the spermatheca of this species did contain sperm, a high percentage was dead. While the spermatheca of M. pruinosa has such atrophic tendencies, males, have developed long and spiny lateral processes similar to those of C. cornelia. This suggests functions other than spermathecal sperm removal. The lateral processes possibly function as stoppers or guides for manipulating the aedeagal head to remove the sperm mass from the bursa.

雌雄同体中的雌性大坝自我有两个精子储存器官，一个球形囊囊和一个管状精子；雄性具有带有弯曲头部的奇特雌雄同体，可以去除囊囊精子和侧面的刺突，以去除精子精子。然而，横向过程在物种或种群之间的宽度相对于精子导管而异。较窄的过程在物理上能够访问精子精子，而较宽的精子则无法访问精子。在这项研究中，比较了具有不同精子结构，calopteryx cornelia和mnais pruinosa的两个仅具有精子数量（数量）（数量），而且使用最近开发的基于现场死亡/死亡的方法基于现场死亡/死亡的方法的精子数量（数量），比较了具有不同精子结构，calopteryx Cornelia和Mnais pruinosa之间的两个仅具有精子结构的毛ther虫物质之间的精子。 Calopteryx Cornelia是一种典型的精子精子去除剂。在该物种中，囊囊精子和精子精子之间的生存力相似。另一方面，在M. Pruinosa中，精子比Bursa小得多，通常没有精子。即使该物种的精子确实包含精子，也有很高的比例死亡。虽然pruinosa的精子具有如此萎缩的趋势，但雄性已经发展出与C. Cornelia类似的长长而多刺的横向过程。这表明除精子精子去除以外的功能。横向过程可能充当阻止者或指南，以操纵副体的头部以从Bursa中去除精子质量。

项目成果

日本産カワトンボ属の分類的,生態的諸問題への新しいアプローチ(2)資料

日本蜻蜓分类学和生态问题的新方法（2）材料

• 发表时间: 2005
• 期刊: Aeschna 42(印刷中)
• 作者: 林 文男;土畑重人;二橋 亮
• 通讯作者: 二橋 亮

Distribution patterns of two damselfly species. Mnais costalis and M. strigata, in the Boso Peninsula, Chiba Prefecture

两种豆娘的分布格局。

• 发表时间: 2004
• 期刊: Tombo, (In Japanese) 47
• 作者: Futahashi;R.;Hayashi;F.
• 通讯作者: F.

日本産カワトンボ属の分類的,生態的諸問題への新しいアプローチ(1)総論

日本蜻蜓目分类学和生态问题的新方法（一）一般讨论

• 发表时间: 2004
• 期刊: Aeschna 41
• 作者: 林 文男;土畑重人;二橋 亮
• 通讯作者: 二橋 亮

A new approach to resolve the taxonomic and ecological problems of Japanese Mnais damselflies (Odonata: calopterygidae)

解决日本豆娘分类学和生态问题的新方法（蜻蜓目：calopterygidae）

• 发表时间: 2005
• 期刊: (2) References and examined specimens, Aeschna, (In Japanese) 42 (in press)
• 作者: Hayashi;F.;Dobata;S.;Futahashi;R.
• 通讯作者: R.

房総半島(千葉県)におけるオオカワトンボとカワトンボの分布様式

房总半岛（千叶县）巨蜻蜓和河蜻蜓的分布格局

• 发表时间: 2004
• 期刊: Tombo 47
• 作者: 二橋 亮;林 文男
• 通讯作者: 林 文男