Morphologically divergent sperm in Odonata: phylogenetic patterns and relations with sperm competition

蜻蜓目精子形态分化：系统发育模式及其与精子竞争的关系

• 批准号: 13640632
• 负责人: HAYASHI Fumio
• 金额: $ 1.92万
• 依托单位: Tokyo Metropolitan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13640632/
• 关键词: insect; sperm; sperm competition; molecular phylogeny; copulation; 精子競走

In this study, the shape and size of sperm were compared among about 150 species of damselflies and dragonflies (Insecta: Odonata). All sperm observed in damselflies had a usual form as seen in most other insects; however, those observed in dragonflies were atypical, aflagellar or bundled. Changes from normal sperm to aflagellar sperm seemed to occur once on their possible phylogenetic tree. Changes from normal sperm to bundled sperm also occurred once phylogenetically. Genital structure conspicuously differed between the closely related species of damselflies, and this suggested that the genital structure is influenced by some selection pressure in mating behavior. Detailed phylogenetic analyses were conducted for Japanese Mnais and Trigomphus species, based on DNA sequences. The species belonging to each genus were clearly separated by the nuclear DNA, but not by mitochondrial DNA. The incongruent phylogenetic conclusions based on mtDNA and nDNA suggests that introgressive hybridization had occurred between the closely related species in the past. In such a situation, avoidance of hybridization, that is reinforcement of reproductive isolation, may play an important role to advance speciation.

在这项研究中，比较了大约150种的豆腐和蜻蜓（昆虫：odonata）的精子的形状和大小。在大多数其他昆虫中所见，在大坝中观察到的所有精子都具有通常的形式。但是，在蜻蜓中观察到的那些是非典型，黄曲黄色或捆绑的。从正常的精子到Aflagellar精子的变化似乎发生在其可能的系统发育树上。从正常的精子到捆绑的精子的变化也发生在系统发育上。生殖器结构在密切相关的豆腐物种之间显着差异，这表明生殖器结构受交配行为的某些选择压力的影响。基于DNA序列，对日本MNAI和Trigomphus物种进行了详细的系统发育分析。属于每个属的物种被核DNA明显分离，而不是通过线粒体DNA分离的。基于mtDNA和NDNA的系统发育结论不一致，这表明过去与密切相关的物种之间发生了介绍性杂交。在这种情况下，避免杂交（加强生殖隔离）可能在推进物种方面起重要作用。

项目成果

Hayashi, F.: "Morphological divergence in insect sperm"Hakumon. 53 (7). 6-13 (2001)

Hayashi, F.：“昆虫精子的形态分歧”Hakumon。

林 文男: "昆虫の精子の不思議な世界"白門. 53・7. 6-13 (2001)

林文雄：“昆虫精子的神秘世界”Shiromon 53・7（2001）。