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New NMR methods developed for structure determination of large proteins

New NMR methods developed for structure determination of large proteins
开发用于大蛋白质结构测定的新核磁共振方法
批准号:
24657074
负责人:
KOJIMA Chojiro
金额:
$2.58万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2012
资助国家:
日本
项目状态:
已结题
起止时间:
2012-04-01 至 2014-03-31

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中文摘要
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英文摘要
NMR structure can be determined for proteins with molecular weight less than 50 kDa, however, no structure for beyond 100 kDa. In this research, we have developed a new NMR method to analyze very large proteins. This method consists of sensitivity enhancement of 13C direct detection, and 13C assignment and analysis procedures employed for solid state NMR. These methods were applied to florigen receptor with molecular mass beyond 50 kDa.
期刊论文(63)
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会议论文
DOI: 10.2142/biophysics.7.51
发表时间: 2011
期刊: Biophysics (Nagoya-shi, Japan)
影响因子: --
作者: [Sudo Y, Tanaka R, Kobayashi T, Kamo N, Kohno T, Kojima C]
通讯作者: Kojima C
DOI: 10.1093/nar/gkr951
发表时间: 2012-01
期刊: Nucleic acids research
影响因子: 14.9
作者: [Kawahara I, Haruta K, Ashihara Y, Yamanaka D, Kuriyama M, Toki N, Kondo Y, Teruya K, Ishikawa J, Furuta H, Ikawa Y, Kojima C, Tanaka Y]
通讯作者: Tanaka Y
A novel sensitivity enhancement method for molecules possessing long longitudinal relaxation time
一种新颖的长纵向弛豫时间分子灵敏度增强方法
DOI: --
发表时间: 2012
期刊:
影响因子: --
作者: [Kyoko Furuita, Yoshikazu Hattori, Takahisa Ikegami, Toshimichi Fujiwara, Chojiro Kojima]
通讯作者: Chojiro Kojima
植物花成ホルモン・フロリゲンの受容と機能のメカニズム
植物开花激素florogen的接收机制及作用
DOI: --
发表时间: 2012
期刊:
影响因子: --
作者: [辻寛之, 田岡健一郎, 大木出, 玉置祥二郎, 中島千佳, 中村洋之, 稲田のりこ, 児嶋長次郎, 島本功]
通讯作者: 島本功
52
    Protein structure analysis in human cell using advanced in-cell NMR techniques
    • 批准号:
      20H03191
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $11.32万
    • 财政年份:
      2020
    • 负责人:
      KOJIMA Chojiro
    • 依托单位:
    Development of fully-automated system for NMR structural analysis of higher molecular weight proteins
    • 批准号:
      17H03641
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $11.15万
    • 财政年份:
      2017
    • 负责人:
      KOJIMA Chojiro
    • 依托单位:
    NMR live imaging technology to monitor membrane protein structure
    • 批准号:
      23370053
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $12.81万
    • 财政年份:
      2011
    • 负责人:
      KOJIMA Chojiro
    • 依托单位:
    Structural bases of spliceosome indepemdent splicing
    • 批准号:
      20370038
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $13.15万
    • 财政年份:
      2008
    • 负责人:
      KOJIMA Chojiro
    • 依托单位:
    海外基金