NMR live imaging technology to monitor membrane protein structure
NMR live imaging technology to monitor membrane protein structure
批准号:
23370053
负责人:
KOJIMA Chojiro
金额:
$12.81万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011-04-01 至 2014-03-31
中文摘要
点击翻译按钮获取中文摘要
英文摘要
Membrane protein is an important target for drug discovery. In this study, using solid state NMR, we have developed a method to detect a signal from a target membrane protein expressed on the cell surface. Special isotope labeling technique enabled to suppress the background signals and enhanced the signal-to-noise ratio. Finally we succeeded to detect NMR signals of the target membrane protein using live cells.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
登录
查看更多内容
^<1>H, ^<15>N and ^<13>C resonance assignments of the conserved region in the middle domain of S. pombe Sin1 protein.
粟酒裂殖酵母Sin1蛋白中间结构域保守区的^ 1 H、^ 15 N和^ 13 C共振归属。
DOI:
10.1007/s12104-014-9550-6
发表时间:
2014
期刊:
Biomolecular NMR Assignments
影响因子:
0.9
作者:
[Kataoka S, Furuita K Hattori Y, Kobayashi N, Ikegami T, Shiozaki K, Fujiwara T, Kojima C]
通讯作者:
Kojima C
Double- Acquisition Method and Lysine ^<13>C- Methylation NMR
双采集法和赖氨酸13C-甲基化NMR
DOI:
--
发表时间:
2013
期刊:
影响因子:
--
作者:
[Saori Kataoka, Kyoko Furuita, Yoshikazu Hattori, Naohiro Kobayashi, Takahisa Ikegami, Kazuhiro Shiozaki, Toshimichi Fujiwara and Chojiro Kojima, 児嶋長次郎, 児嶋長次郎, Chojiro Kojima, 児嶋長次郎]
通讯作者:
児嶋長次郎
A novel sensitivity enhancement technique for solution NMR utilizing long longitudinal relaxation time
利用长纵向弛豫时间的新型溶液核磁共振灵敏度增强技术
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Kyoko Furuita, Yoshikazu Hattori, Takahisa Ikegami, Toshimichi Fujiwara, Chojiro Kojima]
通讯作者:
Chojiro Kojima
NMR滴定実験と蛋白質の化学修飾
核磁共振滴定实验和蛋白质的化学修饰
DOI:
--
发表时间:
2013
期刊:
NMR
影响因子:
--
作者:
[Ken-ichiro Taoka, Izuru Ohki, Hiroyuki Tsuji, Chojiro Kojima and Ko Shimamoto, 古板恭子・服部良一・児嶋長次郎]
通讯作者:
古板恭子・服部良一・児嶋長次郎
DOI:
10.2142/biophysics.9.21
发表时间:
2013
期刊:
Biophysics (Nagoya-shi, Japan)
影响因子:
--
作者:
[Abe-Yoshizumi R, Kobayashi S, Gohara M, Hayashi K, Kojima C, Kojima S, Sudo Y, Asami Y, Homma M]
通讯作者:
Homma M
共 40 条
Protein structure analysis in human cell using advanced in-cell NMR techniques
-
批准号:20H03191
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$11.32万
-
财政年份:2020
-
负责人:KOJIMA Chojiro
-
依托单位:
Development of fully-automated system for NMR structural analysis of higher molecular weight proteins
-
批准号:17H03641
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$11.15万
-
财政年份:2017
-
负责人:KOJIMA Chojiro
-
依托单位:
New NMR methods developed for structure determination of large proteins
-
批准号:24657074
-
项目类别:Grant-in-Aid for Challenging Exploratory Research
-
资助金额:$2.58万
-
财政年份:2012
-
负责人:KOJIMA Chojiro
-
依托单位:
Structural bases of spliceosome indepemdent splicing
-
批准号:20370038
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$13.15万
-
财政年份:2008
-
负责人:KOJIMA Chojiro
-
依托单位:
Structural basis of IRE1 mRNA splicing mechanism
-
批准号:18570106
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$2.57万
-
财政年份:2006
-
负责人:KOJIMA Chojiro
-
依托单位:
海外基金