Reproduction barrier in interploidy crosses in Vitis

葡萄属间倍体杂交的繁殖障碍

• 批准号: 24658025
• 负责人: TAO Ryutaro
• 金额: $ 2.66万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658025/
• 关键词: 果樹; ブドウ; 倍数性; ゲノムインプリンティング; 次世代シークエンシング技術; 受粉受精; 胚発生; 胚乳発生; 次世代シークエンス技術

Seed formation after inter- and intra- ploidy crosses were investigated in Vitis. Crosses were made between a European grape cultivar Rosaki (V. vinifera), a rootstock cultivar 5BB (V. riparia x V. berlandieri), and chromosome doubled clones produced from Rosaki and 5BB by colochicine treatment. Differences in endosperm cells were observed among crosses 30 days after flowering. Accumulation of protein bodies in endosperm at this stage correlated with the seed germination ability after seed maturation. RNA-Seq analysis was performed with developing seeds. Stress related gene expressions were high in inter-ploidy crosses.

研究了葡萄属植物倍性间和倍性内杂交后的种子形成.在欧洲葡萄栽培品种Rosaki（V. vinifera）和砧木栽培品种5 BB（V. riparia x V. berlandieri）之间进行杂交，并通过秋水仙素处理从Rosaki和5 BB产生染色体加倍的克隆。开花后30天观察到胚乳细胞的差异。这一时期胚乳中蛋白体的积累与种子成熟后的萌发能力有关。用发育中的种子进行RNA-Seq分析。逆境胁迫相关基因在倍性间杂交中表达量较高。