Molecular mechanisms of gametophytic self-incompatibility in Prunus species

李属配子体自交不亲和性的分子机制

• 批准号: 09460018
• 负责人: TAO Ryutaro
• 金额: $ 5.95万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-09460018/
• 关键词: S-RNase; sweet cherry; Japanese apricot; Japanese plum; almond; ア-モンド

Prunus, one of the genera in the Rosaceae, includes important commercial fruit and nut tress species. Most of the Prunus species exhibit the monofactorial gametophytic self-incompatibility system which is controlled by a single locus, the S-locus, with multiple alleles. As Prunus species are unable to bear fruit parthenocarpically, they will not set fruit unless pollinated with cultivars with different S-genotypes. Thus, determination of correct pollen incompatibility groups and assignment of cultivars to the groups are essential for good crop production.This study identifies S-RNases associated with self-incompatibility in sweet cherry, almond, Japanese plum, and Japanese apricot. cDNAs encoding the S-RNases have been cloned and DNA-sequenced, which leads to the development of a molecular typing system for S-alleles in these fruit species. Furthermore, S-RNase allele that can be used as a molecular marker for self-compatibility in Japanese apricot has been explored.

李属（Prunus）是蔷薇科（Rosaceae）的一个属，是重要的经济果树和坚果树种。大多数李属植物的配子体自交不亲和系统都是由一个S位点控制的多等位基因单因子系统。李属植物不能单性结实，除非与不同S基因型的品种授粉，否则不会坐果。因此，正确的花粉不亲和性组的确定和分配的品种组是必不可少的良好的作物production.This研究确定S-RNases与甜樱桃，杏仁，日本李，日本杏自交不亲和性。编码S-RNases的cDNA已被克隆和DNA测序，这导致了这些水果物种中S-等位基因的分子分型系统的发展。此外，还探索了可作为杏自交亲和性分子标记的S-RNase等位基因。

项目成果

Tao,R. et al.: "Molecular typing of S alleles through identification, characterization and cDNA cloning for S-RNases in sweet cherry"J.Amer.Soc.Hort.Sci.. 124. 224-233 (1999)

陶，R.

Tao, R.et al.: "Identification of stylar RNase associated with gametophytic self-incompatibility in almond (Prunus dulcis)"Plant Cell Physiology. 38. 304-311 (1997)

陶，R.等人：“与杏仁（Prunus dulcis）配子体自交不亲和性相关的花柱核糖核酸酶的鉴定”植物细胞生理学。

山根 久代: "Identification and cDNA clonling for S-Rnases in self-incompatible Japanese plum (Prunus salicina Lindl cv Sordur)"Plant Biotechnology. 16・5. 389-396 (1999)

Hisayo Yamane：“自交不亲和的日本李子（Prunus salicina Lindl cv Sordur）中 S-Rnas 的鉴定和 cDNA 克隆”植物生物技术 16・5（1999）。

Tao,R. et al.: "Identification of stylar RNases associated with gametophytic self incompatibility in almond (Prunus dulcis)"Plant Cell Physiology. 38. 304-311 (1997)

陶，R.

Tamura,M.,K.Ushijima,H.Sassa,H.Hirano,R.Tao,et al.: "Identification of self-incompatibility genotypes of almond by allele-specific PCR analysis"Theoretical Applied Genetics. 101. 344-349 (2000)

Tamura,M.,K.Ushijima,H.Sassa,H.Hirano,R.Tao,et al.：“通过等位基因特异性 PCR 分析鉴定杏仁自交不亲和性基因型”理论应用遗传学。