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Identification of the pollen determinant in the gametophytic self-incompatibility system through the analysis of self-compatible mutants in Prunus

通过李属自交亲和突变体分析鉴定配子体自交不亲和系统中的花粉决定因素

基本信息

批准号：
13460014
负责人：
TAO Ryutaro
金额：
$ 8.51万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13460014/
关键词：
stone fruit S-RNase gametophytic self-incompatibility sweet cherry Japanese apricot almond Japanese plum peach

项目摘要

This study identifies the pollen determinant (pollen S) of the S-RNase based gametophytic self-incompatibility system utilizing the mutated S haplotypes. Based on the results obtained we have developed the working model for the molecular basis of self-(in)compatibility in Prunus. The summary of the results obtained is as follows.(1)Analysis of the mutated S haplotype in almondComparisons of the S locus region of a mutated Sc-hapotype with the original one identifies S haplotype-specific F-box progein gene (SFB). SFB shows the S haplotype-specific sequence polymorphism and pollen-specific expression, indicating that SFB is a very good candidate for the pollen Sin Prunus.(2)Analysis of self-compatible S haplotypes of cherry and Japanese apricotSf of Japanese apricot and S4' of sweet cherry are known to be the pollen part mutant S haplotypes. Analysis of their S locus regions revealed insertion mutations in SFBs of these S haplotypes. The insertions lead to frame shift to produce truncated transcripts for SFB, which could be the cause of self-compatibility in Sf and S4' haplotypes.(3)Self-(in)compatibility in tetraploid sour cherryIn Solanaceae, which shows the same type of gametophytic self-incompatibility as Prunus, polyploidization generally leads to self-compatibility through the so-called phenomenon, "the competitive interaction". We have analyzed self-(in)compatibility in the progeny of several crosses between several sour cherry cultivars. It appeared that the segregation of self-(in)compatibility is determined by the number of functional and non-functional S haplotypes present in the progeny, suggesting that "the competitive interaction" is absent in Prunus.(4)Working model for the molecular basis of self-(in)compatibility in PrunusBased on the above-described results, we have developed and published the working model for the genetic and molecular basis of self-(in)compatibility in Prunus.

本研究利用突变的S单倍型，鉴定了基于S- rnase的配子体自交不亲和系统的花粉决定因子（花粉S）。在此基础上，我们建立了李属植物自相容性的分子基础工作模型。所得结果总结如下。(1)杏仁突变S单倍型分析将突变S单倍型的S位点区域与原S单倍型进行比较，鉴定出S单倍型特异性F-box progein基因（SFB）。SFB表现出S单倍型特异性序列多态性和花粉特异性表达，表明SFB是一个很好的花粉候选者。(2)樱桃和杏子自交亲和S单倍型分析：已知杏子的tsf和甜樱桃的S4′为花粉部分突变S单倍型。对其S位点区域的分析显示，这些S单倍型的sfb中存在插入突变。插入导致帧移位，产生SFB的截断转录本，这可能是Sf和S4单倍型自亲和性的原因。(3)四倍体酸樱桃的自交不亲和性。茄科酸樱桃与李子具有同种配子体自交不亲和性，其多倍体化一般通过所谓的“竞争互作”现象导致自交不亲和性。对几个酸樱桃品种杂交后代的自交亲和性进行了分析。自（内）亲和的分离似乎是由后代中存在的功能性和非功能性S单倍型的数量决定的，这表明“竞争相互作用”在Prunus中不存在。(4)李属植物自交亲和的分子基础工作模型基于上述结果，我们建立并发表了李属植物自交亲和的遗传和分子基础工作模型。