权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Molecular Basis of Self-incompatibility in Fruit Tree Species in Prunus

李属果树自交不亲和性的分子基础

基本信息

批准号：
17380021
负责人：
TAO Ryutaro
金额：
$ 8.9万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380021/
关键词：
almond apricot cherry Japanese apricot plum SFB stone fruit S-RNase 自家不和合性

项目摘要

It has been known that the specificity of the self-incompatibility system in Prunus is determined by the pistil- and pollen-determinants, S-RNase (S-ribonuclease) and SFB (S haplotype-specific F-box protein), respectively; however, the molecular mechanism underlying the self-incompatibility reaction is still unclear. This study was conducted to elucidate the molecular basis of the gametophytic self-incompatibility system in Prunus (Rosaceae). To this end, we cloned and sequenced the Slocus region of several self-compatible mutant S haplotypes of cherries, almonds, Japanese apricots, and Japanese plums. It appeared that most of the self-compatible S haplotypes encode truncated dysfunctional SFB, indicating that mutation in SFB confers self-compatibility in Prunus. Molecular cloning of the self-compatible peach also indicated that SC in peach is conferred by mutation in its SFB. These observations are in contrast to the solanaceous self-incompatibility system, in which dysfunction in the pollen-determinant leads to self- and cross-incompatibilities. Furthermore, genetic and molecular analyses using the tetraploid sour cherry demonstrated that the presence of two different pollen S alleles in a pollen grain does not confer self-compatibility in Prunus. Namely, the well-known phenomenon of competitive interaction is ablsent in Prunus, as opposed to the solanaceous system. Based on these results, we have proposed the one-allele match model to explain the molecular basis of self-incompatibility system in Prunus.

已知李树自交不亲和系统的特异性分别由雌蕊和花粉决定因子S- rnase （S-核糖核酸酶）和SFB （S单倍型特异性F-box蛋白）决定；然而，自不相容反应的分子机制尚不清楚。本研究旨在阐明蔷薇科李树配子体自交不亲和系统的分子基础。为此，我们克隆了樱桃、杏仁、日本杏和日本李的几个自亲和突变S单倍型的Slocus区域并对其进行了测序。大多数自亲和的S单倍型编码截断的功能失调的SFB，表明SFB的突变赋予了李的自亲和。自交亲和性桃的分子克隆也表明，桃的SC是通过其SFB突变获得的。这些观察结果与茄属植物的自交不亲和系统相反，在自交不亲和系统中，花粉决定因子的功能障碍导致自交和交叉不亲和。此外，利用四倍体酸樱桃进行的遗传和分子分析表明，在一个花粉粒中存在两个不同的花粉S等位基因并不会导致李的自亲和性。也就是说，与茄属植物系统相反，李属植物中没有众所周知的竞争相互作用现象。在此基础上，我们提出了单等位基因匹配模型来解释李树自交不亲和系统的分子基础。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

A simple and rapid procedure for the detection of self-compatible individuals in Japanese apricot (Prunus mume Sieb. et Zucc. ) using loop-mediated isothermal amplification (LAMP) method

使用环介导等温扩增 (LAMP) 方法简单快速地检测日本杏 (Prunus mume Sieb. et Zucc.) 中的自交亲和个体

DOI：
发表时间：
2006
期刊：
HortScience 41
影响因子：
0
作者：
Habu;T.;et al.
通讯作者：
et al.

Molecular markers for the self-compatible S^4-haplotype

自亲和 S^4 单倍型的分子标记

DOI：
发表时间：
2005
期刊：
影响因子：
0
作者：
Tao;R.;et al.
通讯作者：
et al.

Characterization of SLFL1, a pollen-expressed F-box gene located in the Prunus S locus

DOI：
10.1007/s00497-008-0069-9
发表时间：
2008-06-01
期刊：
SEXUAL PLANT REPRODUCTION
影响因子：
0
作者：
Matsumoto, Daiki;Yamane, Hisayo;Tao, Ryutaro
通讯作者：
Tao, Ryutaro

A Low Transcriptional Level of Se-RNase in the Se -haplotype Confers Self-compatibility in Japanese Plum

DOI：
10.21273/jashs.132.3.396
发表时间：
2007-05
期刊：
Journal of the American Society for Horticultural Science
影响因子：
1.9
作者：
A. Watari;T. Hanada;H. Yamane;T. Esumi;R. Tao;H. Yaegaki;M. Yamaguchi;K. Beppu;I. Kataoka
通讯作者：
A. Watari;T. Hanada;H. Yamane;T. Esumi;R. Tao;H. Yaegaki;M. Yamaguchi;K. Beppu;I. Kataoka

S^e-haplotype confers self-compatibility in Japanse plum (Prunus salicina Lindl. )

S^e 单倍型赋予日本李子（Prunus salicina Lindl.）自交亲和性