Development of IgE mimotope-antibody fusion derivatives specific for sensitized allergens

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House dust mite allergy is a serious social problem, and establishment of its curable therapy is an urgent issue also in our country. Objective of this study is to develop basic technologies for the generation of sensitized allergen-specific IgE mimotope-human IgG antibody constant region (Fcgamma) chimeric molecules for patient-tailored specific immunotherapy against dust mite allergy. We first developed a new and highly-reproducible method for identification of the IgE mimotope peptides by introducing a pre-screening procedure using allergen-specific IgG from mite-allergic patients. We also developed basic technical strategies for production of recombinant IgE mimotope-Fcgamma chimeras using animal cell expression systems.