Exhaustive Allergenome Analysis of Japanese Cedar Pollen Allergen Molecules

日本雪松花粉过敏原分子的详尽过敏基因组分析

• 批准号: 17607008
• 负责人: ONO Kazuhisa
• 金额: $ 2.37万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17607008/
• 关键词: allergen; cedar pollinosis; allergenome analysis; 感作アレルゲン; IgE頻度マップ; アレルゲン封入多角体; プロテアーゼ; リパーゼ; ハイドロラーゼ; IgE反応頻度マップ; β-1,3-glucanase; aspartyl protease; 次世代診断システム; テーラーメイド医療

Japanese cedar pollinosis is now a nation-wide allergic disease, although our knowledge about the causing factors, i.e. repertoire of Japanese cedar pollen (JCP) allergen molecules, is still limited. Objective of this study is exhaustive identification and characterization of JCP allergens via proteomics in conjunction with molecular immunological strategies, which we call "Allergenome Analysis".(1) Mapping of JCP allergensTwo-dimensional (2D) IgE immunoblotting revealed that; 1) total of 131 allergen spots were found on the 2D map, and major allergens Cry j 1 and Cry j 2 spots distributed as multiple isoforms. 2) IgE-binding profile of each pollinosis patients was quite different from each other, suggesting that tailor-made molecular diagnosis is crucial for effective immunotherapy. 3) 31 allergen spots showed higher IgE binding frequency than that of Cry j 2 (40%).(2) Identification and immunochemical characterization of new major JCP allergensWe next tried structural and immunochemical analyses of above new CJP allergens using TOF-MS analysis and cDNA cloning. We first identified class IV chitinase homologue (CJP4) as a novel major allergen with 100% IgE binding frequency, which is higher than that of Cry j 1 (71%). We also cloned two other allergens homologous to β-1,3-glucanase (CPA39) and aspartyl protease/nucleoid DNA binding protein family (CPA63), and functionally expressed those recombinant allergens using baculovirus-insect cell culture system. Intriguingly, we found that CJP4 and CPA39 showed IgE crossreactivity with other plant species such as latex or olive pollen, suggesting that JCP chitinase and/or β-1,3-glucanase may serve as pan-allergens involved in the pathogenesis of oral allergy syndrome.

日本雪松花粉症现在是一种全国性的过敏性疾病，虽然我们的知识，即日本雪松花粉（JCP）过敏原分子的剧目，仍然是有限的。本研究的目的是通过蛋白质组学结合分子免疫学策略，我们称之为“过敏基因组分析”，对JCP过敏原进行详尽的鉴定和表征。(1)JCP变应原的二维免疫印迹分析结果显示：1）在2D图谱上共发现131个变应原点，主要变应原Cry j 1和Cry j 2以多种亚型分布。2)每个花粉症患者的IgE结合谱彼此差异很大，这表明量身定制的分子诊断对于有效的免疫治疗至关重要。3)31个变应原点的IgE结合率高于Cry j 2（40%）。(2)新的主要JCP变应原的鉴定和免疫化学表征接下来，我们尝试使用TOF-MS分析和cDNA克隆对上述新的CJP变应原进行结构和免疫化学分析。我们首先鉴定了IV类几丁质酶同源物（CJP 4）作为一种新的主要过敏原，其IgE结合频率为100%，高于Cry j 1（71%）。我们还克隆了另外两个与β-1，3-葡聚糖酶（CPA 39）和乙酰化蛋白酶/类核DNA结合蛋白家族（CPA 63）同源的变应原，并利用杆状病毒-昆虫细胞培养系统进行了功能性表达。有趣的是，我们发现CJP 4和CPA 39与其他植物物种如乳胶或橄榄花粉显示IgE交叉反应性，表明JCP几丁质酶和/或β-1，3-葡聚糖酶可能作为泛过敏原参与口腔变态反应综合征的发病机制。

项目成果

免疫治療におけるスギ花粉抗原反復注射とダニ抗原反復注射によるTh1/Th2細胞応答の違いについて

免疫治疗中重复注射雪松花粉抗原与重复注射蜱抗原的Th1/Th2细胞反应差异

• 发表时间: 2006
• 期刊: アレルギー科 21
• 作者: KANAI;Toshiyuki;林 鷹治
• 通讯作者: 林 鷹治

Dietary pulverized konjac glucomannan suppresses scratching behavior andskin inflammatory immune response inNC/Nga mice.

膳食魔芋葡甘聚糖粉可抑制 NC/Nga 小鼠的抓挠行为和皮肤炎症免疫反应。

• 发表时间: 2007
• 期刊: International Archives of Allergy and Immunology 114
• 作者: Shinohara;H. and Kurosaki;T.;Nobukazu Onishi
• 通讯作者: Nobukazu Onishi

A newly identified class IV chitinase allergen from Japanese cedar pollen shows IgE crossreactivity with latex C-serum

来自日本雪松花粉的新鉴定的 IV 类几丁质酶过敏原显示出与乳胶 C 血清的 IgE 交叉反应性

• 发表时间: 2005
• 期刊: The XIXth World Allergy Organization Congress (Allergy & Clin.Immunol.Int.) Suppl.1
• 作者: T.Ishibashi;K.Inoue;H.Gotanda;K.Kumamaru;斎藤文彦;T.Fujimura et al.
• 通讯作者: T.Fujimura et al.

スギ花粉由来の新規アレルゲン

源自雪松花粉的新型过敏原

• 发表时间: 2005

プロテオーム解析によるスギ花粉・ダニアレルゲンの全容解明

通过蛋白质组分析彻底阐明雪松花粉和螨虫过敏原

• 发表时间: 2006
• 期刊: アレルギー・免疫 13
• 作者: Nomura T;et al.;Ando T.et al.;河本正次
• 通讯作者: 河本正次