Application of mite allergen to desensitization immunotherapy

具有广泛交叉反应性的纯化抗原的组合似乎比单一纯化抗原更适合于脱敏治疗。纯化抗原组分已被用于免疫治疗的第一步，其是基于强皮肤反应性和对抗血清和对螨过敏的患者血清的高反应性从螨全培养物中分离的，并且已被证明具有高治疗效果。此外，该级分没有引起副反应、哮喘诱导和过敏反应，因此该级分被认为是具有高安全性和有效性的治疗性抗原。我们继续分析患者免疫球蛋白或抗体亚类的滴度变化之间的关系，我们还分离了低分子量（LM）抗原（4k）和高分子量（HM）抗原，（150- 155 k）作为其它特征性抗原。 关于我们 结果表明，HM抗原组分与LM抗原组分具有良好的免疫原性和激活T细胞的能力，HM抗原组分与LM抗原组分具有良好的免疫原性，HM抗原组分与LM抗原组分具有良好的免疫原性和激活T细胞的能力，HM抗原组分与LM抗原组分具有良好的免疫原性，HM抗原组分与LM抗原组分具有良好的免疫原性，HM抗原组分与LM抗原组分具有良好的免疫原性和激活T细胞的能力，HM抗原组分与LM抗原组分具有良好的免疫原性，HM抗原组分与LM抗原组分具有良好的免疫原性和激活T细胞的能力，HM抗原组分与LM抗原组分具有良好的免疫原性，HM抗原组分与LM抗原组分具有良好的免疫原性和激活T细胞的能力，HM抗原组分与LM抗原组分具有良好的免疫原性和免疫原性，HM抗原组分与LM抗原组分具有良好的免疫原性和激活T细胞的能力，HM抗原组分与LM抗原组分具有良好的免疫原性和激活T细胞的能力，HM抗原组分具有良好的免疫原性和免疫原性。但不诱导过敏反应。抗原组分可用于免疫治疗。然而，从不同批次的螨培养物中制备大量组成恒定、效价稳定的治疗性抗原，方法：通过cDNA交叉杂交，将螨变应原的cDNA克隆分为9类，其中表达产物Mag 1（高活化小鼠T细胞的能力），Mag 44（原肌球蛋白）和Mag 3（高活化人和小鼠T细胞的能力），以及具有较低IgE结合活性的Mag 29（hsp 70）和Mag 124。少

The combination of purified antigens with broader cross-reactivity, rather than single purified antigen, seemed to be suitable for desensitization therapy.A purified antigen fraction has been used at first step of the immunotherapy, which was isolated from a whole mite culture on the basis of potent skin reaction and high reactivity to antisera and patients' sera allergic to mite, and has proved highly therapeutic effects.Moreover, the fraction has elicited no side reaction, induction of asthma and anaphylaxis reaction, so that the fraction has been recognized as a therapeutic antigen with high safety and efficiency.Continuously, we are analyzing relationship between changes in titers of patients' immunoglobulins or antibody subclasses, or in sensitivity of basophils to allergens and the efficacy of the immunotherapy.We also isolated low-molecular-size (LM) antigen (4k) and high-molecular-size (HM) antigens (150-155k) as other characteristic antigens.The LM anttigen with potentially as … More thma-inducible property which is facilely permeable to mucosa cross-reacted with the fraction effective for immunotherapy.The antigen would be fit for structual analysis of mite-allergy specific epitopes and induction of T cell anergy.On the other hand, the HM antigen fractions cross-reactive to the LM antigen possessed high immunogenicity and ability to activate T cells, but not induced anaphylaxis reaction.The antigen fractions might be used for immunotherapy.However, it seemed very difficult to prepare a large quantity of therapeutic antigen with constant composition and stable potency from different batches of mite cultures by only biochemical methods.Thus, we isolated cDNA clones encoding mite allergen and classfied the immunoreactive clones into 9 groups by cDNA cross-hybridization.Among them, expression products, Mag1 (high ability to activate mouse T cell), Mag44 (tropomyosin), and Mag3 (high ability to activate human and mouse T cell) with potent IgE-binding activity, and Mag29 (hsp70) and Mag 124 with less IgE-binding activity, were immunobiochemically characterized in this study. Less