Application of mite allergen to desensitization immunotherapy

螨变应原在脱敏免疫治疗中的应用

基本信息

批准号：
05650802
负责人：
ONO Kazuhisa
金额：
$ 1.47万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

The combination of purified antigens with broader cross-reactivity, rather than single purified antigen, seemed to be suitable for desensitization therapy.A purified antigen fraction has been used at first step of the immunotherapy, which was isolated from a whole mite culture on the basis of potent skin reaction and high reactivity to antisera and patients' sera allergic to mite, and has proved highly therapeutic effects.Moreover, the fraction has elicited no side reaction, induction of asthma and anaphylaxis reaction, so that the fraction has been recognized as a therapeutic antigen with high safety and efficiency.Continuously, we are analyzing relationship between changes in titers of patients' immunoglobulins or antibody subclasses, or in sensitivity of basophils to allergens and the efficacy of the immunotherapy.We also isolated low-molecular-size (LM) antigen (4k) and high-molecular-size (HM) antigens (150-155k) as other characteristic antigens.The LM anttigen with potentially as … More thma-inducible property which is facilely permeable to mucosa cross-reacted with the fraction effective for immunotherapy.The antigen would be fit for structual analysis of mite-allergy specific epitopes and induction of T cell anergy.On the other hand, the HM antigen fractions cross-reactive to the LM antigen possessed high immunogenicity and ability to activate T cells, but not induced anaphylaxis reaction.The antigen fractions might be used for immunotherapy.However, it seemed very difficult to prepare a large quantity of therapeutic antigen with constant composition and stable potency from different batches of mite cultures by only biochemical methods.Thus, we isolated cDNA clones encoding mite allergen and classfied the immunoreactive clones into 9 groups by cDNA cross-hybridization.Among them, expression products, Mag1 (high ability to activate mouse T cell), Mag44 (tropomyosin), and Mag3 (high ability to activate human and mouse T cell) with potent IgE-binding activity, and Mag29 (hsp70) and Mag 124 with less IgE-binding activity, were immunobiochemically characterized in this study. Less

纯化的抗原与更广泛的交叉反应性（而不是单个纯化的抗原）的组合似乎适合于脱敏治疗。在免疫疗法的第一步中，已经使用了一种纯化的抗原分数，该抗原分数是从整个螨虫培养中分离出来的，该抗原的基础是在潜在的皮肤反应和高度反应性的抗疗法和患者的高度反应性的基础上，并具有高度反应性，并具有高度的效果。不引起无侧反应，哮喘和过敏反应的诱导，因此，分数已被公认为具有高安全性和效率的治疗性抗原。连续，我们正在分析患者滴答的滴度变化，免疫细胞群或抗体亚群，或对基础属性的敏感性以及近似属性的效率效应。（LM）抗原（4K）和高分子大小（HM）抗原（150-155K）作为其他特征性抗原。具有……更可渗透性的LM抗原具有……更可渗透性，可易于渗透，可与粘膜交叉相互反应，可为免疫疗法而有效分析。另一方面，HM抗原级分与LM抗原潜在的高免疫原性和激活T细胞的能力交叉反应，但诱导过敏反应的能力，抗原级分可用于免疫治疗。既然很难通过与温和抗原的抗原量相互抗衡的量相互抗管，并且很难与温和的抗原量相互抗衡。从cDNA杂交杂交，我们隔离了生化方法。 124在这项研究中具有较少的IgE结合活性，以免疫化学的特征进行免疫化学特征。较少的