Molecular design, synthesis, and molecular mechanism of MALDI-MS matrices

近年来，由于MALDI-MS具有高灵敏度、高通量和低样品消耗的特点，已被用于低分子量代谢物的分析。虽然代谢物主要是有机酸，但由于对常用基质（如9-氨基吖啶（9-AA））的敏感性较差，因此很少采用负电离模式。我们的目的是在阴性模式下合成和评价用于低分子代谢物MALDIMS分析的新基质。合成了包括吖啶、蒽和喹啉衍生物在内的60多个化合物作为MALDI-MS候选基质。利用这些合成基质，用MALDI-TOF-MS在负电离模式下测定了230种低分子量代谢物。发现了几种新的合成基质比9-AA更有效。特别是，氨基蒽衍生物提供了更好的分析物信号，允许51种氨基酸衍生物在负模式下检测。

Recently, MALDI-MS has been used for low-molecular-weight metabolite analysis because it is a highly sensitive, high-throughput, and low sample-consuming technique. Although metabolites are predominantly organic acids, negative ionization mode has rarely been utilized due to poor sensitivity with commonly used matrices such as 9-aminoacridine (9-AA). Our purpose is synthesis and evaluation of new matrices for low-molecular metabolite MALDIMS analysis in negative mode. More than 60 compounds including acridine, anthracene and quinoline derivatives were synthesized as MALDI-MS matrix candidates. Using these synthetic matrices, 230 kinds of low-molecular weight metabolites were measured by MALDI-TOF-MS in negative ionization mode. Several new synthetic matrices were found to be more efficient than 9-AA. Especially, aminoanthracene derivatives provided much better analyte signals allowing 51 kinds of amino acid derivatives to be detected in negative mode.