Analysis of mechanisms underlying strain recognition memory in mice using visualization techniques

通过使用稳定表达绿色荧光蛋白(GFP)的转基因小鼠，在二尖瓣和由交配雄性化学信号和副嗅球(AOB)交配信号关联自然激活的颗粒细胞中，绿色荧光蛋白(GFP)偶联到活性依赖基因c-fos，我们发现在AOB切片中，GFP阳性的二尖瓣细胞以爆发模式激发，而GFP阴性的二尖瓣细胞以紧张模式激发。此外，我们在正常小鼠的AOB切片上发现，激活AOB中二尖瓣细胞上的mGluR2抑制了电压依赖性的钙通道，从而抑制了二尖瓣细胞释放谷氨酸，而血管加压素增强了AOB中二尖瓣到颗粒细胞突触的长期增强诱导，减少了二尖瓣细胞的树突状抑制。

By using transgenic mice that stably express the green fluorescent protein (GFP) coupled to the activity-dependent gene c-fos in mitral and granule cells naturally activated by the association of mating male chemosignals and mating signal in the accessory olfactory bulb (AOB), we found in AOB slices that GFP-positive mitral cells fired in burst mode in contrast to GFP-negative mitral cells that fired in tonic mode. In addition, we found in AOB slices prepared from normal mice that the activation of mGluR2 on mitral cells in the AOB inhibited voltage-dependent Ca2+ channels, thereby suppressing the release of glutamate from the mitral cells and that vasopressin that enhances the induction of long-term potentiation at the mitral-to-granule cell synapse in the AOB reduced dendrodendritic inhibition of mitral cells.