Molecular and Sellular Mechanisms Underlying an Olfactory Memory in the Context of Pregnancy Block

妊娠阻滞背景下嗅觉记忆的分子和细胞机制

• 批准号: 01570087
• 负责人: KABA Hideto
• 金额: $ 1.34万
• 依托单位: Kochi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570087/
• 关键词: Memory; Pheromone; Tuberoinfundibular dopaminergic neuron; Accessory olfactory bulb; Excitatory amino acid receptor; Cholecystokinin receptor; Field potential; Current source-density analysis; 隆起漏斗系ド-パン細胞; 長期増強; 隆起漏斗系ド-パミン神経分泌細胞

Female mice form a long-term olfactory memory to the urinary odors of the male that mates with them. This olfactory memory is essential to their reproductive success. It is now established that the formation of this memory is dependent on neural mechanisms within the accessory olfactory bulb (AOB). The purpose of this study is to examine the synaptic and molecular mechanisms within the AOB for the olfactory memory by infusing various drugs into the AOB. The results are summarized as follow : (1) Blockade of the GABA receptors in the AOB without mating prevents any male from blocking pregnancy following a subsequent mating, suggesting that the GABAergic blockade does create an olfactory memory. (2) Protein kinase inhibition in the AOB during the critical period, but not 6 hours later, prevents memory formation. (3) Protein synthesis inhibition in the AOB in the late phase (3-6hours after mating) but not early phase of the critical period prevents memory formation. These results suggest … More that changes in synaptic efficacy at the dendrodendritic synapse between mitral and granule cells in the AOB are crucial to recognition of the mate's pheromones by female mice.A further purpose of this study is to approach to electrophysiological correlates of this memory. Synaptic transmission in the External Plexiform Layer (EPL) of the AOB following stimulation of the vomeronasal organ was analyzed using a one-dimensional Current Source-Density (CSD) method in anaesthetized female mice. The CSD analysis revealed a major sink (inward membrane current) in the EPL. The sink in the EPL was blocked by the non-NMDA receptor antagonist, CNQX, but not by the NMDA receptor receptor antagonist, D-AP-5, suggesting the mediation of non-NMDA receptors in the mitral to granule cell dendrodendritic synapse.We also demonstrated that the projections of the AOB activate excitatory amino acid receptors in the amygdala and cholecystokinin-B receptors in the medial preoptic area, thereby causing excitation of tuberoinfundibular dopaminergic neurons. Less

雌性小鼠对与其交配的雄性小鼠的尿液气味形成长期的嗅觉记忆。这种嗅觉记忆对于它们的繁殖成功至关重要。现在已经确定这种记忆的形成依赖于副嗅球（AOB）内的神经机制。本研究的目的是通过向 AOB 中注入各种药物来检查 AOB 内嗅觉记忆的突触和分子机制。结果总结如下：(1)在没有交配的情况下阻断AOB中的GABA受体可以防止任何雄性在随后的交配后阻断怀孕，这表明GABA能阻断确实产生了嗅觉记忆。 (2) 在关键时期，但在 6 小时后，AOB 中的蛋白激酶抑制会阻止记忆形成。 (3)AOB中的蛋白质合成抑制在后期(交配后3-6小时)而不是关键期的早期抑制记忆的形成。这些结果表明，AOB 中二尖瓣细胞和颗粒细胞之间的树突突触功效的变化对于雌性小鼠识别配偶信息素至关重要。这项研究的另一个目的是探讨这种记忆的电生理相关性。在麻醉雌性小鼠中，使用一维电流源密度 (CSD) 方法分析了犁鼻器刺激后 AOB 外丛状层 (EPL) 中的突触传递。 CSD 分析揭示了 EPL 中的主要汇（内向膜电流）。 EPL 中的下沉被非 NMDA 受体拮抗剂 CNQX 阻断，但不被 NMDA 受体拮抗剂 D-AP-5 阻断，这表明非 NMDA 受体在二尖瓣到颗粒细胞树突突触中的介导。 内侧视前区的胆囊收缩素-B 受体，从而引起结节漏斗部多巴胺能神经元的兴奋。较少的

项目成果

C.ーS.Li,H.Kaba,H.Saito and K.Seto: "Neural mechanisms underlying the action of primer pheromones in mice" Neuroscience. 36. 773-778 (1990)

C.-S.Li、H.Kaba、H.Saito 和 K.Seto：“小鼠引物信息素作用的神经机制”《神经科学》36. 773-778 (1990)。

H.Kaba,E.B.Keverne,H.Saito and K.Seto: "Importance of the mitral to granule cell clendroclendritic synapse in the accessary olfactory bulb for olfactory memory" Neurosciences. 16. 185-190 (1990)

H.Kaba、E.B.Keverne、H.Saito 和 K.Seto：“二尖瓣对辅助嗅球中颗粒细胞细枝突触对嗅觉记忆的重要性”神经科学。

C. -S. Li, H. Kaba, H. Saito and K. Seto: "Neural mechanisms underlying the action of primer pheromones in mice." Neuroscience. 36. 773-778 (1990)

CS。

H.Kaba,E.B.Keverne,H.Saito and K.Seto: "Importance of the mitral to granule cell dendrodendritic synapse in the accessory olfactory bulb for olfactory memory" Neurosciences. 16. 185-190 (1990)

H.Kaba、E.B.Keverne、H.Saito 和 K.Seto：“二尖瓣对辅助嗅球中颗粒细胞树突突触对嗅觉记忆的重要性”神经科学。

C.-S.Li,H.Kaba,H.Saito and K.Seto: "Neural Mechamisms underlying the action of primer pheromcnes in mice" Neuroscience.

C.-S.Li、H.Kaba、H.Saito 和 K.Seto：“小鼠引物信息素作用的神经机制”神经科学。