Establishment of Plasmodium vivax culture system using red blood cells differentiated iPS cells.

利用红细胞分化的iPS细胞建立间日疟原虫培养系统。

• 批准号: 24659192
• 负责人: YANO Kazuhiko
• 金额: $ 2.5万
• 依托单位: 独立行政法人国立国際医療研究センター
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659192/
• 关键词: 原虫; マラリア; 三日熱マラリア原虫; iPS細胞; 三日熱マラリア; 培養; 感染症; 微生物

The objective of the present study is to establish a culture system of Plasmodium vivax. We aimed to conduct erythroid differentiation from induced pluripotent stem cells (iPS cells) which were then co-cultured with the infected blood of vivax malaria patients. Induction of differentiation into red blood cells of iPS cells was successfully done (following Elizabeth S. et al.2005) and red blasts part of the sphere was reddened after 40 days of culture. Reddened spheres (erythroblast) were prepared for Plasmodium vivax infection by adhesion culture. Unfortunately we could not subject this erythroblast to vivax co-culture because there was no vivax malaria patient currently. Instead, we are attempting to establish of infection system to erythroblast, using cultured falciparum malaria parasites.

本研究的目的是建立一个疟原虫的培养系统。我们的目的是与诱导的多能干细胞（IPS细胞）进行红系分化，然后将其与Vivax疟疾患者的感染血共培养。成功完成了将IPS细胞红细胞分化为IPS细胞的红细胞（伊丽莎白S.等人2005年），在培养40天后，球体的红色一部分被变红了。通过粘附培养物制备了红色的球体（红细胞），以用于疟原虫。不幸的是，由于目前没有病毒疟疾患者，我们无法将这种红细胞的共同培养物进行。取而代之的是，我们正在尝试使用培养的恶性疟疾寄生虫来建立感染系统，从而建立成红细胞。