Analysis of gene expression of Helicobacter pylori VacA

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To define the stimulatory factor of VacA toxin production from Helicobacter pylori, we have examined the culture condition under controlling the concentration of some additions.The liquid culture medium containing 5% bovine serum plus 0.1% cyclodextrin conferred highest bacterial growth and toxin production at 37oC for 72 h-cultivation in ambient air containing 5% CO2. We have also assessed whether bacterial cytosolic protein contributes gene expression and secretion of VacA focusing on interaction of mature and in mature toxin using anti-VacA antibody conjugated column chromatography.