Development ofthe infection and proliferation systemof the 1bgenotype HCV based on the new idea

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We tried the development of the infection and proliferation system of the 1b genotype hepatitis C virus (HCV) using human cultured cell lines. In this study, HCV-positive serum (1b genotype) was added tothe human hepatoma cell line Li23, which was found in 2009 as a cell line permitting the reproduction of the HCV RNA and human hepatoma cell line HuH-7 frequently used all over the world. We performed HCV infection under various conditions, however, we did not succeed to demonstrate an increase of the HCV. As one of the causes, we found that expression level of CLDN1, which was one of the HCV receptors, became very low, and thatthe supplement of CLDN1 expression was necessary.