Regulation of HIV-1 restriction factor Apobec3G by mRNA export factor

HIV-1病毒在长期感染期间引起艾滋病相关疾病症状。我们发现mRNA输出因子GANP作为与胞苷脱氨酶APOBEC 3G（A3 G）相关的宿主因子被掺入到病毒体中。GANP以Rev依赖的方式参与HIV-1 mRNA的输出途径。GANP通过调节病毒RNA代谢与病毒粒子中的HIV-1基因组（g）RNA结合。GANP直接与T细胞中的A3 G相互作用，并且这种相互作用是通过参与HIV-1 gRNA降解的Gag蛋白的独特区域。GANP有效地促进A3 G掺入病毒核心，并增强宿主中的非感染性病毒产生。这一知识将表明开发体内疫苗接种程序的可行性。

HIV-1 viruses cause the AIDS-associated disease symptoms during the long-term infection period. We found that mRNA export factor GANP is encapsidated into the virions as a host factor associated with cytidine deaminase APOBEC3G (A3G). GANP is involved in the HIV-1 mRNA export pathway under the Rev-dependent manner. GANP binds to HIV-1 genomic (g)RNA in the virion by regulating viral RNA metabolism. GANP directly interacts with A3G in T-cells, and the interaction is through a unique region of Gag protein involved in HIV-1 gRNA encapsidation. GANP efficiently promotes the A3G incorporation into the viral core and enhances the non-infectious viral production in the host. This knowledge would suggest the feasibility of developing the in-vivo vaccination procedure.