Absolute Quantification of Transmembrane Protein Shedding and Its Application to the Monitoring of Chronic Inflammation

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Ectodomain shedding of transmembrane proteins is induced by inflammatory factors such as chemokines and cytokines. In order to monitor the progress of chronic inflammation, we attempted to establish a monitoring system of the ectodomain shedding using the combination of an originally synthesized internal standard protein mixture and MRM by mass spectrometry. Then, we successfully accomplished the absolute measurement of transmembrane protein contents in mouse tissues at f-mol levels.